摘要
高良姜(Alpinia officinarum Hance)的根茎是著名的“十大广药”之一,具有较高的药用和食用价值。本研究基于EST-SSR分子标记技术,对采自广东省徐闻县的5种高良姜栽培类型(黑鳞蜂窝姜,白鳞蜂窝姜,鸡姜,牛姜,竹头姜)及采自海南省的2种高良姜栽培类型(文昌高良姜,万宁高良姜)进行遗传多样性分析,以构建不同高良姜栽培类型的EST-SSR数字指纹图谱及鉴别体系。结果表明,对EST-SSR位点的100对引物进行筛选,得到扩增条带清晰且稳定的引物21对,占引物总数的21%。利用21对引物对7种高良姜栽培类型基因组DNA进行扩增共得到239条条带,平均每对引物的扩增条带有11.4条,其中多态性条带204条,占85.36%。对这21个多态性EST-SSR位点进行分析,其中多样性指数(I)平均值为1.06,观察杂合度(Ho)、期望杂合度(He)、Nei基因多样性指数、多态性信息含量(PIC)的平均值均在0.5以上。其中14个EST-SSR位点的PIC值大于0.5,属于高多态性位点;5个EST-SSR位点的PIC值在0.25~0.5,属中度多态性位点。建立不同栽培类型高良姜EST-SSR数字指纹图谱及EST-SSR标记检索表,发现引物P-59可将7种高良姜栽培类型区分为6个类型,而引物P-3可将白鳞蜂窝姜与黑鳞蜂窝姜进行区分。该结果可用于7种高良姜主栽培类型的鉴别,为高良姜品种选育提供重要依据。
The rhizome of Alpinia officinarum Hance is one of the most famous traditional Chinese medicine in Guangdong Province,with high medicinal and edible value.In order to identify the cultivation types of A.officinarum in the main producing areas of China,the EST-SSR digital fingerprints of seven A.officinarum cultivars of were established based on EST-SSR molecular marker technology.5 cultivars(’Beilinfengwojiang’,’Heilinfengwojiang’,’Jijiang’,’Niujiang’,’Zhutoujiang’)were collected in Xuwen County of Guangdong Province and 2 cultivars(’Wenchanggaoliangjiang’,’Wanninggaoliangjiang’)were collected in Hainan Province.We screened100 pairs of primers at the EST-SSR sites and 21 pairs of primers could be used to get clear and stable amplification bands.Using these 21 pairs of primers,we amplified the genomic DNA of 7 A.officinarum cultivars and obtained 239 bands.An average of 11.4 bands was amplified per primer pair,and 204 bands showed polymorphism,accounting for 85.36%.We analyzed these 21 polymorphic EST-SSR loci:the average of the diversity index was 1.06,and the average of the observed heterozygosity,desired heterozygote,Nei gene diversity index,and polymorphic information content(PIC)were greater than 0.5.14 EST-SSR sites with PIC>0.5 are high polymorphism sites,and 5 EST-SSR sites with PIC between 0.25 and 0.5 are moderate polymorphism sites.By establishing the EST-SSR digital fingerprint and EST-SSR markers key of different cultivation types of A.officinarum,we found that the P-59 primer can identify the seven A.officinarum cultivation types into 6 groups,and the P-3 primer can identify’Heilinfengwojiang’and’Bailinfengwojiang’.The results of this study can be used to identify seven cultivation types of A.officinarum and provide important basis for the breeding selection of A.officinarum.
作者
赵全杰
谢腾
胡雨晴
唐晓敏
张春荣
杨全
Zhao Quanjie;Xie Teng;Hu Yuqing;Tang Xiaomin;Zhang Chunrong;Yang Quan(Guangzhou Comprehensive Experimental Station of China Chinese Materia Medica Industry Technology System,Key Laboratory for Production and Development of Cantonese Medicinal Materials Under State Administration of Traditional Chinese Medicine(TCM),Guangdong Provincial Research Center on Good Agricultural Practice and Comprehensive Agricultural Development Engineering Technology of CantoneseM edicinal Materials,Guangdong Pharmaceutical University,Guangzhou,510006)
出处
《分子植物育种》
CAS
北大核心
2023年第2期557-565,共9页
Molecular Plant Breeding
基金
中央本级重大增减支项目(2060302)
国家中药材产业技术体系建设专项(CARS-21)
国家中医药管理局“道地药材生态种植及质量保障项目”
广东药科大学创新训练计划资助项目(202110573011)共同资助。
关键词
高良姜
分子标记
遗传多样性
SSR
Alpinia officinarum Hance
Molecular marker
Genetic diversity
SSR
