摘要
建立一种快速、灵敏的电雾式检测器(CAD)方法,同时测定紫苏子及炒紫苏子配方颗粒中多成分的含量.依照中药配方颗粒的制备要求,利用Acclaim Polar Advantage Ⅱ C_(18)(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈(A)-0.1%三氟乙酸水(B),梯度洗脱,分析泵梯度洗脱条件:0—25 min:20%→55%A,25—26 min:55%→95%A;采用Chromeleon 7.2梯度补偿泵,流速为0.7 mL·min^(−1),进样体积15μL.研究表明,通过CAD检测器可以同时检测紫苏子、炒紫苏子的3种特征性成分,无样品基质干扰,r大于0.999,精密度相对偏差(RSD)分别为1.35%(迷迭香酸-3-O-β-D-葡萄糖苷)、1.19%(3-脱羟基迷迭香酸-3-O-β-D-葡萄糖苷)、1.40%(迷迭香酸).通过对10批不同紫苏子和炒紫苏子配方颗粒含量分析,炒紫苏子的3个特征性酚酸类成分含量高于紫苏子含量,其中炒紫苏子中3-去羟基迷迭香酸-3-O-β-D-葡萄糖苷含量显著高于紫苏子(P=0.017).本方法简便、快速,能同时对紫苏子及炒紫苏子配方颗粒指纹图谱中无标准品的特征性成分进行定量测定.
To establish a rapid and sensitive CAD method for simultaneous determination of multiple components in the formulation particles of perilla seeds and their fried products.The Acclaim Polar Advantage Ⅱ C_(18) column(4.6 mm×250 mm,5μm)was used for analysis.Acetonitrile as eluent A,0.1%trifluoroacetic acid in water as eluent B,and the gradient elution conditions were as follows:0—25 min:20%—55%A;25—26 min:55%—95%A,at a flow rate of 0.7 mL·min^(−1) and injection volume of 15μL.A chromeleon 7.2 gradient compensation pump was used in this method.Three characteristic components of perilla seeds and fried perilla seed could be detected simultaneously by CAD detector,without sample matrix interference,r was greater than 0.999,and the relative deviation(RSD)of precision were 1.35%(rosmarinic acid-3-O-β-D-glucoside),1.19%(3’-dehydroxy rosine-3-O-β-D-glucoside),and 1.40%(rosmarinic acid),respectively.The results showed that the content of three characteristic components in the fried perilla seeds was higher than that in the perilla seeds,and the content of 3’-dehydroxy rosine-3-O-β-D-glucoside in the fried perilla seeds was significantly higher than that in the perilla seeds(P=0.017).The method is simple,rapid and can be used for the quantitative determination of the characteristic components in the fingerprint of perilla seed formula granules.
作者
邸继鹏
赵艺晨
彭倩
章军
陈莎
刘安
DI Jipeng;ZHAO Yichen;PENG Qian;ZHANG Jun;CHEN Sha;LIU An(Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,100700,China;Thermo Fisher Scientific(China)Co.,Ltd,Beijing,100007,China)
出处
《环境化学》
CAS
CSCD
北大核心
2023年第1期341-344,共4页
Environmental Chemistry
基金
中国中医科学院科技创新工程(CI2021A04515)资助。
关键词
炒紫苏子
电雾式检测器
配方颗粒
多成分
含量测定.
fried perilla seeds
charged aerosol detector
formula particles
multi-component
content determination.
