摘要
目的 研究血清miR-95、血清miR-200a-3p在变应性鼻炎(allergic rhinitis,AR)患者中的表达及其与病情严重程度相关性分析。方法 选取2019年3月至2021年5月温州市中西医结合医院收治的AR患者90例作为研究组,另收集90例同期在温州市中西医结合医院体检的健康受试者作为对照组。采用鼻部症状总评分(total nasal symptom score,TNSS)和单个鼻部症状评分(individual nasal symptom score,INSS)对AR的严重程度进行评估。对比两组肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素(interleukin,IL)-1β、IL-4、IL-6、IL-8、IL-10、IL-13、IL-17、miR-95、miR-200a-3p水平。结果 研究组血清miR-95水平高于对照组,血清miR-200a-3p水平低于对照组,差异有统计学意义(P<0.05)。受试者操作特征曲线分析结果显示,血清miR-95的曲线下面积(area under curve,AUC)为0.857,95%置信区间(confidence interval,CI):0.821~0.911,血清miR-200a-3p的AUC为0.861,95%CI:0.809~0.908。血清miR-95的截断值为1.25,对AR患病风险预测的特异性为69.3%,敏感度为75.4%。血清miR-200a-3p的截断值为1.02,对AR患病风险预测的特异性为71.2%,敏感度为77.9%。两组血清miR-95均与miR-200a-3p呈负相关(P<0.05)。AR患者血清中miR-95m水平与患者鼻塞、鼻痒、TNSS评分均呈正相关(P<0.05);AR患者血清中miR-200a-3p水平与患者鼻塞、鼻痒、TNSS评分均呈负相关(P<0.05);血清miR-95、miR-200a-3p水平与AR患者的喷嚏和流涕评分不具有明显的相关性(P>0.05)。研究组患者血清miR-95水平与TNF-α、IL-4、IL-6、IL-13、IL-17水平呈正相关(P<0.05),与IL-10水平呈负相关(P<0.05)。研究组患者血清miR-200a-3p水平与TNF-α、IL-6、IL-8、IL-13、IL-17水平呈负相关(P<0.05),与IL-10呈正相关(P<0.05)。结论 变应性鼻炎患者血清中miR-95呈现高表达,miR-200a-3p呈现低表达,miR-95水平与患者病情严重程度呈正相关,miR-200a-3p水平与患者病情严重程度呈负相关。
Objective To study the expression of serum miR-95 and miR-200a-3p in patients with allergic rhinitis(AR) and analyze their correlation with the severity of the disease. Methods A total of 90 patients with AR treated in Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine from March 2019 to May 2021 were selected as the research group, and another 90 healthy subjects who were examined in Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine at the same time were selected as the control group. Total nasal symptom score(TNSS) and individual nasal symptom score(INSS)were used to evaluate the severity of AR. The levels of tumor necrosis factor-α(TNF-α), interleukin(IL)-1β, IL-4, IL-6, IL-8,IL-10, IL-13, IL-17, miR-95, miR-200a-3p were compared between the two groups. Results The level of serum miR-95 in the study group was significantly higher than that in the control group, and the level of miR-200a-3p was significantly lower than that in the control group(P<0.05). The area under curve(AUC) of serum miR-95 was 0.857, 95% confidence interval(CI):0.821-0.911, and the AUC of serum miR-200a-3p was 0.861, 95%CI: 0.809-0.908. The cut-off value of serum miR-95 was 1.25,with a specificity of 69.3% and a sensitivity of 75.4% for predicting the risk of AR. The cut-off value of serum miR-200a-3p was 1.02, with a specificity of 71.2% and a sensitivity of 77.9% for predicting the risk of AR. Serum miR-95 in the two groups was negatively correlated with miR-200a-3p(P<0.05). The level of miR-95 in serum of patients with AR was positively correlated with nasal congestion, nasal itching and TNSS score(P<0.05). Serum miR-200a-3p level in patients with AR was negatively correlated with nasal congestion, nasal itching and TNSS score(P<0.05). Serum miR-95 and miR-200a-3p levels were not significantly correlated with the sneezing and runny nose scores of AR patients(P>0.05). The level of serum miR-95 in the study group was positively correlated with the levels of inflammatory factors TNF-α, IL-4, IL-6, IL-13 and IL-17(P<0.05). There was a negative correlation with the level of IL-10(P<0.05). The level of serum miR-200a-3p was negatively correlated with the levels of inflammatory factors TNF-α, IL-6, IL-8, IL-13 and IL-17(P<0.05), but positively correlated with IL-10(P<0.05).Conclusion The expression of miR-95 in the serum of patients with allergic rhinitis is high, but that of miR-200a-3p is low. The level of miR-95 is positively correlated with the severity of the patient’s illness, while the level of miR-200a-3p is negatively correlated with the severity of the patient’s illness.
作者
麻琼钒
杜慧慧
郑国君
MA Qiongfan;DU Huihui;ZHENG Guojun(Department of Ear-Nose-Throat,Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine,Wenzhou 325000,Zhejiang,China)
出处
《中国现代医生》
2023年第2期70-74,共5页
China Modern Doctor
