摘要
为确定重庆烟区烟草叶片上出现黄化斑点、枯斑等症状的病原,进而制定出科学的综合防治措施,对采自重庆市涪陵和巫溪烟区与北碚试验田的18份烟叶样品进行了病毒种类检测与鉴定。根据病毒外壳蛋白基因序列设计了特异性引物,采用RT-PCR扩增及BLAST序列对比分析,结果表明:涪陵烟区烟叶上出现黄化斑点、枯斑等症状的样品中检测出烟草花叶病毒(Tobacco mosaic virus,TMV)和辣椒脉斑驳病毒(Chilli veinal mottle virus,ChiVMV),检出率分别为54.55%和100%;巫溪烟区样品中检测出TMV和马铃薯Y病毒(Potato virus Y,PVY),检出率分别为100%和66.67%;北碚烟草试验田病叶样品中检测出ChiVMV,检出率为100%。其中,ChiVMV为重庆地区烟草上首次发现。
In order to clarify the pathogens that causing symptoms of yellowing spots and necrotic spots in tobacco from Chongqing tobacco-growing area and to develop a comprehensive control strategy,18leaf samples were collected from Fuling and Wuxi tobacco-growing areas and Beibei experimental fields.Specific primers were designed according to the gene sequence of the virus coat proteins,and RT-PCR amplification and BLAST sequence alignment analyses were conducted.The results showed that Tobacco Mosaic Virus(TMV)and Chilli veinal mottle virus(ChiVMV)were detected from the samples with yellowing spots and necrotic spots in Fuling tobacco-growing area,and the detection rates were 54.55%and 100%respectively.TMV and Potato Virus Y(PVY)were detected from the samples in Wuxi tobacco-growing area,and the detection rates were 100%and 66.67%respectively.ChiVMV was detected from the diseased leaf samples in Beibei tobacco-growing experimental fields,and the detectable rate was 100%.ChiVMV was detected from tobacco leaves in Chongqing for the first time.
作者
田绍锐
温玉霞
马婷
陈海涛
徐宸
汪代斌
孙现超
TIAN Shaorui;WEN Yuxia;MA Ting;CHEN Haitao;XU Chen;WANG Daibin;SUN Xianchao(College of Plant Protection,Southwest University,Chongqing 400715,China;Chongqing Tobacco Science Research Institute,Chongqing 400715,China)
出处
《烟草科技》
CAS
CSCD
北大核心
2023年第1期33-38,共6页
Tobacco Science & Technology
基金
国家自然科学基金面上项目“一个以S.l SYTA为中心的互作链影响番茄花叶病毒侵染致病的机制研究”(31870147)、“烟草IP-L在番茄花叶病毒侵染移动过程中的作用及作用机制研究”(31670148)
中国烟草总公司重庆公司科技项目“重庆烟草叶斑病传播阻断与保护预防技术研究”(B20211-NY1315)、“烟草病害靶向免疫控病技术研究应用与产品研发”(A20201NY02-1306)、“纳米硒在重庆烤烟生产中的应用效果评价及配套技术研究”(B20202NY1338)
