摘要
采用顶空固相微萃取和气相色谱-质谱联用技术,比较不同涂层纤维萃取头和不同色谱柱对细叶韭花水提液易挥发性成分萃取分离的效果和特点。结合本室前期试验结果,共检测出133类易挥发性物质,包括10种含硫类、22种酯类、8种酸类、46种碳氢类、15种醇类、8种酮类、10种醛类、8种酚类、2种醚类和4种其他类化合物。其中细叶韭花水提液关键性香气成分包括对乙烯基愈疮木酚、1,3-二噻烷、二甲基三硫醚、二甲基二硫醚、甲基丙基二硫醚、2,5-二甲基噻吩、3,4-二甲基噻吩等物质。根据总离子流色谱图,细叶韭花水提液易挥发性成分在HP-5色谱柱上的分离效果优于RTX-WAX色谱柱。同时,比较不同萃取头在检出成分数量、总相对含量的差异,结合主成分分析筛选出最优萃取头,结果表明采用85μm PA萃取头,在HP-5色谱柱上分离鉴定效果较好;采用65μm PDMS/DVB萃取头,在RTX-WAX色谱柱上分离鉴定效果较好。
Headspace solid-phase microextraction and gas chromatography-mass spectrometry were used to compare the effects and characteristics of different coated fiber extraction heads and different chromatographic columns on the extraction and separation of volatile components in the water extract of Allium tenuissimum flowers. Combined with the laboratory’s preliminary test results,a total of 133 types of volatile substances were detected,including 10 types of sulfur,22 types of esters,8 types of acids,46 types of hydrocarbons,15 types of alcohols,8 types of ketones,10aldehydes,8 phenols,2 ethers and 4 other compounds. Among them,the key aroma components of the water extract of Allium tenuissimum flowers included p-vinyl guaiacol,1,3-dithiane,dimethyl trisulfide,dimethyl disulfide,methyl propyl disulfide,2,5-dimethylthiophene,3,4-dimethylthiophene and other substances. According to the total ion chromatogram,the separation effect of the volatile components in the water extract of Allium tenuissimum flowers with HP-5 chromatographic column was better than that with RTX-WAX chromatographic column. At the same time,the differences of the number of components and the total relative content detected by different extraction heads were compared,and the optimal extraction head was selected by principal component analysis,the results showed that,85μm PA extraction head had better separation and identification effects with HP-5 chromatographic column;65μmPDMS/DVB extraction head had better separation and identification effects with RTX-WAX chromatographic column.
作者
邹熙
丁鹏霞
李美萍
ZOU Xi;DING Pengxia;LI Meiping(College of Life Science,Shanxi University,Taiyuan 030006)
出处
《中国食品添加剂》
CAS
北大核心
2023年第2期246-254,共9页
China Food Additives
关键词
顶空固相微萃取
气相色谱-质谱联用
细叶韭花提取液
挥发性成分
headspace solid-phase microextraction(HS-SPME)
gas chromatography-mass spectrometry(GCMS)
aqueous extract of Allium tenuissimum flowers
volatile components
