摘要
选取甲烷排放通量较高并且受喹诺酮类抗生素(QNs)污染较严重的白洋淀湿地为研究对象,通过开展13C标记的微宇宙实验,研究典型QNs包括:氧氟沙星(OFL)、诺氟沙星(NOR)和氟甲喹(FLU)对白洋淀湿地DAMO过程的短期影响.结果表明:3种QNs均促进了微生物DAMO过程,且对DAMO的促进效果表现为OFL>NOR>FLU;除添加2ng/g的OFL对DAMO反应速率潜势的影响基本可以忽略外,添加其他浓度(20,200,2000ng/g)的OFL均促进了白洋淀湿地的DAMO过程,并且这种促进作用总体呈现出剂量效应;进一步分析QNs对NO_(2)^(-),NO_(3)^(-)分别驱动的DAMO的影响发现,200ng/g的NOR抑制了由细菌驱动的以NO_(2)^(-)为电子受体的DAMO过程(nitrite-DAMO),但更大程度地促进了由古菌驱动的以NO_(3)^(-)为电子受体的DAMO过程(nitrate-DAMO),最终表现为QNs对NO_(3)^(-)和NO_(2)^(-)共同驱动的总体的DAMO过程的促进作用.
The Baiyangdian wetland,with a high methane emission flux and heavily contaminated with quinolone antibiotics(QNs),was selected as the study area.The short-term effects of typical QNs including Ofloxacin(OFL),Norfloxacin(NOR),and Flumequine(FLU)on denitrifying anaerobic methane oxidation(DAMO)processes in Baiyangdian wetland was investigated by conducting 13C-labeled microcosm experiments.The results showed that:All three QNs promoted the microbial DAMO process,and the promotion effect on DAMO was OFL>NOR>FLU;Except for 2ng/g OFL,which had a negligible effect on the DAMO reaction rate,additional concentrations(20,200,2000ng/g)all promoted the DAMO processes in Baiyangdian wetlands,and the promotion showed a dose effect in general;Further analysis of the effects of QNs on the nitrite-DAMO driven by NO_(2)^(-)and the nitrite-DAMO driven by NO_(3)^(-)respectively,it could be found that 200ng/g NOR inhibited the bacterial-driven DAMO process with NO_(2)^(-)as electron acceptor(nitrite-DAMO),but promoted to a greater extent the archaea-driven DAMO process with NO_(3)^(-)as electron acceptor(nitrate-DAMO),ultimately showing a facilitation effect of QNs on the overall DAMO process driven by both NO_(3)^(-)and NO_(2)^(-).
作者
杨玉琦
赵悦文
张秋兰
刘长礼
文萌托
YANG Yu-qi;ZHAO Yue-wen;ZHANG Qiu-lan;LIU Chang-li;WEN Meng-tuo(Fujian Provincial Key Laboratory of Water Cycling and Eco-Geological Processes,Xiamen 361021,China;School of Water Resources and Environment,China University of Geosciences,Beijing 100083,China;Institute of Hydrogeology and Environmental Geology,Chinese Academy of Geological Sciences,Shijiazhuang 050061,China;State Key Laboratory of Biogeology and Environmental Geology,China University of Geosciences,Wuhan 430074,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2023年第2期631-637,共7页
China Environmental Science
基金
河北省自然科学基金资助项目(C2020504001)
厦门市自然科学基金资助项目(3502Z20227158)
中国地质科学院水文地质环境地质研究所基本科研业务费资助项目(SK202108)。
