摘要
目的:研究葡萄籽提取物(GSE)、维生素C(Vc)、葡萄籽维生素C胶囊对D-半乳糖模型小鼠的抗氧化作用。方法:除空白对照组小鼠,其余小鼠腹腔注射D-半乳糖连续6周后,按造模后小鼠的丙二醛(MDA)水平值随机分为模型组、Vc低、高剂量组(8.3、16.7 mg/kg·bw),GSE低、高剂量组(8.3、16.7 mg/kg·bw)、葡萄籽Vc胶囊低、高剂量组(25、50 mg/kg·bw)共7组,在连续给药30天后,测定小鼠脑、肝及血清中的丙二醛(MDA)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)及超氧化物歧化酶(SOD)的水平。结果:与模型组相比,Vc高剂量组、GSE高剂量组、葡萄籽Vc胶囊低、高剂量组小鼠的肝脏系数降低,脾脏系数升高(P <0.01,P <0.05),葡萄籽Vc胶囊高剂量组的胸腺系数显著升高(P<0.01);维生素C高剂量组、葡萄籽提取物(GSE)高剂量组、葡萄籽维生素C胶囊高剂量组均能使小鼠各组织中的GSH、GSH-PX和SOD的值增加,使MDA值减少,且与模型组差异显著(P <0.01,P <0.05)。结论:证明维生素C、葡萄籽提取物及葡萄籽维生素C胶囊能恢复D-半乳糖衰老模型小鼠的氧化损伤,与其提高机体抗氧化酶活性有关。
Objective:To study effects of grape seed extract(GSE),vitamin C(Vc)and grape seed vitamin C capsule on antioxidant ability in D-galactose model mice. Methods:Except blank control group,other mice were intraperitoneal injected with d-galactose for 6 weeks. Based on MDA level,mice were randomly divided into model group,low-dose vitamin C group,high-dose vitamin C group(8.3,16.7 mg/kg · bw),grape seed extract lowdose and high-dose groups(8.3,16.7 mg/kg·bw),grape seed vitamin C capsule low-dose and high-dose groups(50,100 mg/kg·bw). The content of malondialdehyde(MDA),reduced glutathione(GSH),glutathione peroxidase(GSH-PX)and antioxidant dismutase(SOD)levels in brain,liver and blood were determined after 30 days of continuous administration. Results:Compared with model group,liver coefficient was decreased and spleen coefficient was increased in Vc high-dose group,GSE high-dose group,grape seed Vc capsule low-dose group and high-dose group(P < 0.01,P < 0.05). The thymus coefficient of grape seed Vc capsule high-dose group was significantly increased(P < 0.01). The values of GSH,GSH-Px and SOD in high-dose vitamin C group,highdose grape seed extract(GSE)group and high and low-dose grape seed vitamin C capsule group were increased,while the value of MDA was decreased. The differences were significant compared with the model group(P < 0.01,P < 0.05). Conclusion:Vitamin C,grape seed extract and grape seed vitamin C capsule can restore the oxidative damage of d-galactose aging mice and improve antioxidant enzyme activity.
作者
张梓萱
郭佳琦
朱晨辉
ZHANG Zixuan;GUO Jiaqi;ZHU Chenhui(School of Chemical Engineering,Northwest University,Xi’an 710069;Biotech.&Biomed.Research Institute,Northwest University,Xi’an 710069;Shaanxi Medical Device Quality Inspection Institute,Xianyang 712046)
出处
《中国食品添加剂》
CAS
北大核心
2023年第1期208-213,共6页
China Food Additives
基金
国家自然科学基金项目(22178287)。
