摘要
黄酮类化合物杨梅素(myricetin,MYR)具有抗肿瘤、抗氧化等多种药理作用,但在免疫调节方面的作用尚未完全了解,因此本实验通过建立环磷酰胺(cyclophosphamide,CTX)免疫低下小鼠模型来研究MYR对免疫功能的影响。通过称重小鼠脾脏和胸腺以及计算指数评价MYR对模型小鼠症状的缓解程度,用MTT法、溶血试验、酶联免疫吸附法等免疫学相关实验技术检测T、B淋巴细胞增殖,T细胞亚群分布情况,血清溶血素含量,抗体形成细胞数以及细胞因子白介素-2、白介素-4、白介素-6、干扰素-γ的分泌水平。实验数据表明,MYR能提高小鼠免疫器官脾脏、胸腺指数,促进T淋巴细胞和B淋巴细胞的分泌,提高细胞因子水平和抗体形成细胞数量。结果表明MYR能在免疫反应中起积极作用,调节免疫细胞和细胞因子之间的相互作用恢复机体免疫力的平衡,改善免疫力低下的症状。
The flavonoid myricetin(MYR)has antitumor,antioxidant and other pharmacological effects,but its role in immune regulation is not fully understood.Therefore,the effect of MYR on immune function was investigated by establishing a immune mouse model of cyclophosphamide(CTX).The relief degree of symptoms of myricetin was evaluated by weighing spleen and thymus and calculation index.The proliferation of T and B lymphocytes,the distribution of T cell subpopulations,the content of serum hemolysin,the number of antibody forming cells and the secretion levels of cytokines interleukin-2,interleukin-4,interleukin-6,interferon-γwere detected by MTT assay,hemolysis test,enzyme-linked immunosorbent assay and other immunological techniques.Experimental data show that myrictin can improve the mouse immune organ spleen,thymus index,promote the secretion of T lymphocytes and B lymphocyte cells,improve the level of cytokines and antibody forming cells.The results show that myrictin plays a positive role in the immune response,regulate the interaction between immune cells and cytokines to restore the balance of immunity,improve the symptoms of low immunity.
作者
宋博翠
陈倩
李宇琦
李爽
申雪
佟春玉
SONG Bo-cui;CHEN Qian;LI Yu-qi;LI Shuang;SHEN Xue;TONG Chun-yu(College of Life Science&Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第1期33-39,共7页
Natural Product Research and Development
基金
黑龙江省大学生创新创业训练计划(XC2022034)
黑龙江省博士后启动基金计划(LBH-Q21158)
黑龙江八一农垦大学校培育课题(XDB-2016-22)
黑龙江省重点研发计划(GZ20210101)。
关键词
免疫抑制
杨梅素
环磷酰胺
淋巴细胞
immunoregulatory
myricetin
cyclophosphamide
lymphocyte
