摘要
[目的]分析ESR1、TENM1和THEM4单核苷酸多态性(SNPs)与山羊繁殖性状的相关性。[方法]采用MassARRAY SNP分型技术对ESR1、TENM1和THEM4 SNPs位点进行分型,检测其在云上黑山羊、济宁青山羊和辽宁绒山羊3个群体中的遗传学特征,并对其多态性与山羊繁殖性状(产羔数、初生窝重、断奶窝重)进行相关性分析。[结果]群体遗传学分析结果表明,ESR1 g.76097125C>T位点在济宁青山羊和辽宁绒山羊中为低度多态(PIC<0.25),在云上黑山羊中为中度多态(0.25<PIC<0.50);ESR1 g.76139051A>G在云上黑山羊、济宁青山羊和辽宁绒山羊中为中度多态(0.25<PIC<0.50)。TENM1 g.17333655C>T和g.17372494A>G位点在3个山羊群体中表现为低度多态(PIC<0.25);TENM 1 g.17236451G>C和g.17586866T>C位点在3个山羊群体中的多态性(PIC)为0.05~0.36。THEM4 g.101275605G>A位点在3个山羊群体中为低度多态(PIC<0.25)。卡方检验结果表明,ESR1 g.76139051 A>G、THEM4 g.101275605G>A位点在云上黑山羊、济宁青山羊和辽宁绒山羊中处于Hardy-Weinberg平衡状态(P>0.05)。相关分析结果表明,ESR1 g.76097125C>T位点CT和TT型山羊的初生窝重显著高于CC型(P<0.05);TENM1 g.17236451G>C位点GC型山羊的产羔数和断奶窝重显著高于GG型(P<0.05),g.17333655C>T位点CC型山羊产羔数显著高于CT型(P<0.05);其余位点多态性与云上黑山羊产羔数、初生窝重和断奶窝重无显著相关(P>0.05)。[结论]TENM1 g.17236451G>C位点可作为云上黑山羊产羔数和断奶窝重选择的潜在分子标记;TENM1 g.17333655C>T位点可作为云上黑山羊产羔数选择的潜在分子标记;ESR1 g.76097125C>T位点可作为云上黑山羊初生窝重选择的潜在分子标记。该研究结果可为山羊分子育种和标记辅助选择提供理论基础。
[Objective]To analyze correlation between single nucleotide polymorphisms(SNPs)of ESR1,TENM1 and THEM4 and the reproductive traits of goats.[Method]SNPs loci of ESR1,TENM1 and THEM4 were typed by using MassARRAY SNP typing technology,and their genetic characteristics in three populations(Yunshang Black Goat,Jining Grey Goat and Liaoning Cashmere Goat)were detected.The correlation between their polymorphism and the reproductive traits(litter size,litter weight at birth,litter weight at weaning)of goats were analyzed.[Result]The population genetic results showed that ESR1 g.76097125C>T locus was low polymorphic(PIC<0.25)in Jining Grey Goat and Liaoning Cashmere Goat,and it was moderately polymorphic(0.25<PIC<0.50)in Yunshang Black Goat.ESR1 g.76139051A>G was moderately polymorphic(0.25<PIC<0.50)in Yunshang Black Goat,Jining Grey Goat and Liaoning Cashmere Goat.TENM1 g.17333655C>T and g.17372494A>G loci were low polymorphic(PIC<0.25)in the three goat populations.The polymorphism of TENM1 g.17236451G>C and g.17586866T>C loci was 0.05-0.36 in the three goat populations.THEM4 g.101275605G>A locus was low polymorphic(PIC<0.25)in the three goat populations.The chi-square test showed that ESR1 g.76139051A>G and THEM4 g.101275605G>A loci were in Hardy-Weinberg equilibrium(P>0.05)in Yunshang Black Goat,Jining Grey Goat and Liaoning Cashmere Goat.The results of correlation analysis showed that the litter weight at birth of the goats with CT and TT genotypes in ESR1 g.76097125C>T locus was significantly higher than that with CC genotype(P<0.05).The litter size and litter weight at weaning of the goats with GC genotype in TENM1 g.17236451G>C locus were significantly higher than those with GG genotype(P<0.05),the litter size of the goats with CC genotype in g.17333655C>T locus was significantly higher than that with CT genotype(P<0.05).The polymorphism of the other loci had no significant correlation with the litter size,litter weight at birth,litter weight at weaningin Yunshang Black Goat(P>0.05).[Conclusion]TENM1 g.17236451G>C locus could be used as a potential molecular marker for selecting the litter size and litter weight at weaning in Yunshang Black Goat.TENM1 g.17333655C>T locus could be used as a potential molecular marker for the selection of the litter size in Yunshang Black Goat.ESR1 g.76097125C>T locus could be used as a potential molecular marker for selecting the litter weight at birth in Yunshang Black Goat.The results of this study could provide the theoretical basis for the molecular breeding and marker-assisted selection in goats.
作者
李昆谕
刘玉芳
陶林
江炎庭
欧阳依娜
储明星
洪琼花
LI Kun-yu;LIU Yu-fang;TAO Lin(Yunnan Academy of Animal Husbandry and Veterinary Sciences,Kunming,Yunnan 650224;School of Life Sciences and Food Engineering,Hebei University of Engineering,Handan,Hebei 056001;Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture and Rural Affairs/Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193)
出处
《安徽农业科学》
CAS
2023年第1期79-84,共6页
Journal of Anhui Agricultural Sciences
基金
国家现代农业产业技术体系资助项目(CARS-38)
云南省重大科技专项计划项目(202102AE090039)
云南省万人计划——产业技术领军人才项目
中国农业科学院科技创新工程项目(CAAS-ZDRW202106,ASTIP-IAS13)
河北省自然科学基金青年项目(C2019402293)。
