摘要
[目的]通过优化培养基提高异养蛋白核小球藻CP9的生物量产率、蛋白质以及叶绿素含量。[方法]采用5因素8水平的均匀设计法优化小球藻异养培养基的配方,以葡萄糖、KNO_(3)、MgSO_(4)·7H_(2)O以及Fe-EDTA和CaCl_(2)母液为自变量,分析对小球藻生长、蛋白质含量和叶绿素含量的影响,来确定培养基自变量因素的最佳使用浓度。[结果]得到最佳培养基配方为:葡萄糖73.21 g/L、KNO_(3)6.25 g/L、MgSO_(4)·7H_(2)O 0.79 g/L、Fe-EDTA母液13.01 mL/L、CaCl_(2)母液4.80 mL/L。经培养基优化后将蛋白核小球藻CP9的最高细胞密度、蛋白质含量和叶绿素含量分别从11.08 g/L、43.3%和39.42 mg/g提升至15.08 g/L、60.0%和45.06 mg/g。[结论]通过正交优化蛋白核小球藻的异养培养基,将生物量提高了36%,同时藻细胞含有更高的蛋白质(60.0%)和叶绿素含量(45.06 mg/g),可为开发动物饲料或人类食品蛋白质。
[Objective]To enhance the biomass productivity,cells protein and chlorophyll proportions of Chlorella pyrenoidosa CP9 treated by heterotrophic medium optimization.[Method]Uniform design method was used for medium optimization of Chlorella heterotrophic cultivation.A project of 5 factors and 8 levels was designed,that glucose,KNO_(3),MgSO_(4)·7 H_(2)O,Fe-EDTA and CaCl_(2)for the independent variable factors.And the effects on microalgal growth,cells protein and chlorophyll content were analyzed to determine the optimal concentration of different factors.[Result]The obtained medium was that glucose 73.21 g/L,KNO_(3)6.25 g/L,MgSO_(4)·7 H_(2)O 0.79 g/L,Fe-EDTA solution 13.01 mL/L,CaCl_(2)solution 4.80 mL/L.In this case,the microalgal biomass,the proportion of protein and chlorophyll content of Chlorella attained 15.08 g/L,60%and 45.06 mg/g,respectively.[Conclusion]Comparing with original medium,the biomass of Chlorella pyrenoidosa CP9 cultivated in optimization medium had enhanced 36%with high protein and chlorophyll which could develop for the animal feed or human food protein.
作者
李林声
陈涵
周有彩
陈必链
LI Lin-sheng;CHEN Han;ZHOU You-cai;CHEN Bi-lian(Fuqing King Dnarmsa Spriulina Co.,Ltd.,Fuqing 350300,China;School of Life Science,Fujian Normal University,Fuzhou 350117,China)
出处
《生物技术》
CAS
2022年第6期704-709,共6页
Biotechnology
基金
产业链协同创新-海洋生物类产业项目“十三五”海洋经济创新发展示范项目子课题“海洋微藻高值化产品开发及产业链建设”(FZHJ04)。
关键词
均匀设计
异养培养
蛋白核小球藻
生物量
蛋白质
叶绿素
Uniform design
heterotrophic cultivation
Chlorella pyrenoidosa
biomass
protein
chlorophyll
