摘要
目的 研究20例ABO疑难血型标本的基因型并鉴定其分子生物学特性。方法 应用标准血型血清学鉴定技术分析标本的血清学表型。应用聚合酶链反应(polymerase chain reaction, PCR)扩增标本ABO基因7个外显子,对PCR产物进行直接测序,分析ABO疑难血型的基因分型和序列。结果 20例ABO疑难血型标本的血清学表型分别为:A抗原减弱5例、A抗原减弱合并抗-A15例、A抗原正常合并抗-A12例、B抗原减弱8例,基因型分别为:Ax02/O01 3例、Ael07/O01 2例、B313/O01 2例、A204/O02、A220/O01、Ael07/O02、Ael02/O01、Ael02/O02、Ax03/O01、Ax03/O02、B313/O02、B302/O01、B302/O02、Bw19/O02、A102/B313、A101/Bw37各1例。结论 ABO基因分型技术可精准鉴定疑难标本的血型,提供明确的血型遗传学信息,保障临床用血安全。
Objective To study the genotypes of ABO ambiguous blood group samples(n=20) and identify their molecular biological characteristics. Methods The serological phenotype of the samples was analyzed by serological techniques. Seven exons of ABO gene were amplified by polymerase chain reaction(PCR) and the PCR products were directly sequenced;the genotypes and sequences of ABO subtypes were analyzed. Results The serological phenotypes of 20 samples presenting ABO ambiguous blood group were as follows: weak A antigen(n=5), weak A antigen combined with anti-A1 antibody(n=5), normal A antigen combined with anti-A1 antibody(n=2), weak B antigen(n=8). The genotypes of them were as follows: Ax02/O01(n=3), Ael07/O01(n=2), B313/O01(n=2), A204/O02(n=1), A220/O01(n=1), Ael07/O02(n=1), Ael02/O01(n=1), Ael02/O02(n=1), Ax03/O01(n=1), Ax03/O02(n=1), B313/O02(n=1), B302/O01(n=1), B302/O02(n=1), Bw19/O02(n=1), A102/B313(n=1) and A101/Bw37(n=1). Conclusion ABO genotyping technology can accurately identify the ambiguous blood group of samples, provide definite genetic information of blood group and ensure the safety of clinical transfusion.
作者
林秋燕
张进萍
黄震宇
黄清华
范丽萍
付丹晖
黄豪博
LIN Qiuyan;ZHANG Jinping;HUANG Zhenyu;HUANG Qinghua;FAN Liping;FU Danhui;HUANG Haobo(Department of Blood Transfusion,Fujian Medical University Union Hospital,Fuzhou 350001,China;Department of Blood Transfusion,Fuzhou Second Hospital;School of Food and Bioengineering,Fujian Polytechnic Normal University)
出处
《中国输血杂志》
CAS
2023年第1期8-10,共3页
Chinese Journal of Blood Transfusion
基金
福建省科技创新联合资金项目(2018Y9007)。
关键词
ABO血型
疑难血型
基因型
序列分析
ABO blood group
ambiguous blood group
genotype
sequence analysis
