摘要
目的:探索环黄芪醇(Cycloastragenol,CAG)对白癜风的治疗作用及机制。方法:将正常人皮肤黑素细胞PIG1分组如下:对照组、H_(2)O_(2)组、10CAG组、50CAG组和100CAG组,分别使用不同浓度(10、50、100μM)的环黄芪醇和250μM的H_(2)O_(2)与PIG1细胞共培养。通过CCK-8法测定细胞增殖,Annexin V-FITC/PI法检测细胞凋亡,并测定细胞中的黑色素、SOD、CAT和MDA含量。将C57BL/6小鼠随机分为对照组、模型组和CAG组。通过每天涂抹50 mg 40%的莫诺苯宗乳膏建立白癜风小鼠模型,然后使用50 mg/kg的环黄芪醇治疗小鼠。通过HE染色和Masson-Fontana染色检测皮肤组织中的毛囊和黑色素含量。通过Western blotting检测MITF、TYR、TRP-1、TRP-2、Bax、Bcl-2、p-p38/p38的蛋白表达。结果:与H_(2)O_(2)组相比,50CAG组和100CAG组的细胞活力和黑色素含量均升高,凋亡率均降低,Bax蛋白表达水平均降低,而Bcl-2均升高,SOD和CAT水平均升高,而MDA水平均降低(P<0.05)。10CAG组、50CAG组和100CAG组的毛囊和黑色素含量均较H_(2)O_(2)组增多。与H_(2)O_(2)组相比,50CAG组和100CAG组的MITF、TYR、TRP-1、TRP-2和p-p38/p38蛋白表达水平均升高(P<0.05)。与模型组相比,CAG组的的MITF、TYR、TRP-1、TRP-2和p-p38/p38蛋白表达水平均升高(P<0.05)。结论:环黄芪醇在细胞水平和动物水平上均能促进黑色素合成,对白癜风具有较好的治疗作用,其机制可能与p38信号通路的激活有关。
Objective:The purpose of this study was to explore the therapeutic effect and mechanism of cycloastragenol(CAG) on vitiligo.Methods:The normal human skin melanocytes PIG1 were divided into the following groups:control group,H_(2)O_(2) group,10CAG group,50CAG group,100CAG group.PIG1 cells were cultured with different concentrations(10,50,100 μM) of cycloastragenol and250 μM of H_(2)O_(2),respectively.Cell proliferation was detected by CCK-8 method,apoptosis was detected by Annexin V-FITC/PI method,and the contents of melanin,SOD,CAT and MDA in cells were determined.C57BL/6 mice were randomly divided into control group,Model group and CAG group.The vitiligo mouse model was established by applying 50 mg of 40% monobenzone cream daily,and then the mice were treated with 50 mg/kg cycloastragenol.Hair follicles and melanin content in skin tissue were detected by HE staining and Masson-Fontana staining.The protein expressions of MITF,TYR,TRP-1,TRP-2,Bax,Bcl-2,p-p38/p38 were detected by Western blotting.Results:Compared with the H_(2)O_(2) group,the 50CAG group and 100CAG groups had increased cell viability and melanin content,decreased apoptosis rate,decreased Bax protein expression,and increased Bcl-2 protein expression,increased SOD and CAT levels,and decreased MDA levels(P<0.05).The content of hair follicles and melanin in 10CAG group,50CAG group and 100CAG group were higher than those in H_(2)O_(2) group.Compared with the H_(2)O_(2) group,the protein expression levels of MITF,TYR,TRP-1,TRP-2 and p-p38/p38 in the 50CAG group and 100CAG group were all increased(P<0.05).Compared with the model group,the protein expression levels of MITF,TYR,TRP-1,TRP-2 and p-p38/p38 in the CAG group were all increased(P<0.05).Conclusion:Cycloastragenol can promote melanin synthesis at both cellular and animal levels,and has a good therapeutic effect on vitiligo.The mechanism may be related to the activation of p38 signaling pathway.
作者
吴惠林
汪炜
张少君
葛睿
俞文娟
金蝉
周芳
邢倩倩
梁艳
WU Hui-lin;WANG Wei;ZHANG Shao-jun;GE Rui;YU Wen-juan;JIN Chan;ZHOU Fang;XING Qian-qian;LIANG Yan(Department of Dermatology,The Second Affiliated Hospital of Xi’an Medical University,Xian,Shaanxi,710038,China;Skin Hospital,The Second Affiliated Hospital of Xi’an Jiaotong University,Xian,Shaanxi,710014,China;Department of Dermatology and Venereology,The First Affiliated Hospital of Xi’an Jiaotong University,Xi'an,Shaanxi,710061,China)
出处
《现代生物医学进展》
CAS
2023年第1期35-40,46,共7页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81802727)。
