摘要
文章以蛋白核小球藻(Chlorella pyrenoidosa,CP)和富硒蛋白核小球藻(Selenium-enriched Chlorella pyrenoidosa,Se-CP)为原料,对CP、Se-CP的体外抗氧化活性和红细胞氧化损伤的保护作用进行研究。结果表明:经过2 mg/L亚硒酸钠胁迫后,蛋白核小球藻的蛋白质和氨基酸含量、过氧化氢(H2O2)和谷胱甘肽(Glutathione,GSH)含量均显著上升,但丙二醛(Malondialdehyde,MDA)含量显著降低。在体外抗氧化实验中,CP和Se-CP均具有抗氧化能力,相比于CP,Se-CP能显著提高超氧化物歧化酶(Superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)、过氧化氢酶(Catalase,CAT)和抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)的活力,其1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基和超氧阴离子清除率也高于CP。CP与Se-CP处理均能显著抑制2,2’-偶氮二(2-甲基丙基咪)二盐酸盐(2,2’-Azobis(2-Amidinopropane) dihydrochloride,AAPH)诱导的红细胞溶血作用,并提高了红细胞SOD、CAT和GSH-Px酶活力,且Se-CP处理的红细胞的抗氧化物酶活力更高。以上结果表明,蛋白核小球藻在2mg/L的亚硒酸钠胁迫下并未造成小球藻细胞损伤,其体外抗氧化能力显著提高,能明显减缓红细胞的氧化损伤。
In this paper, Chlorella pyrenoidosa(CP) and selenium-enriched Chlorella pyrenoidosa(Se-CP) were used as experimental materials, to explore their in vitro antioxidant activity and protective effects against oxidative stress-induced damage of erythrocytes. The results showed that after 2 mg/L sodium selenite stress, the protein and amino acid content, hydrogen peroxide(H2O2) and glutathione(GSH)content of Chlorella pyrenoidosa increased significantly, but the malondialdehyde(MDA) content decreased significantly. In vitro antioxidant experiments, CP and Se-CP have antioxidant capacity,compared to CP, Se-CP can significantly improve activity of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT) and ascorbate peroxidase(APX), and has a higher clearance of DPPH free radicals, hydroxyl radicals, and superoxide anion. Both CP and Se-CP treatments significantly inhibited the hemolytic effect of erythrocytes induced by AAPH, and increased the activity of erythrocytes SOD, CAT, and GSH-Px, and the antioxidant enzyme activity of Se-CP treated erythrocytes was higher. The above results show that Chlorella cells were not damaged under 2 mg/L sodium selenite stress, and its antioxidant capacity in vitro was significantly improved, which could significantly slow down the oxidative damage of erythrocyte.
作者
邓雨萍
倪婕
张梦菲
周凝
刘小玲
余炼
DENG Yuping;NI Jie;ZHANG Mengfei;ZHOU Ning;LIU Xiaolin;YU Lian(College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;Guilin Normal College,Guilin 541199,China)
出处
《食品科技》
CAS
北大核心
2022年第12期7-14,共8页
Food Science and Technology
基金
广西重点研发计划项目(桂科AB19259010)
广西自然科学基金项目(2020GXNSFAA297038)。
关键词
蛋白核小球藻
富硒
抗氧化
红细胞
Chlorella pyrenoidosa
selenium-enriched
antioxidant
erythrocyte
