摘要
为探讨3种非传统淋巴细胞——恒定自然杀伤T细胞(invariant natural killer T cell,iNKT)、γδT细胞、B1细胞和T细胞、B细胞及NK细胞在慢性HBV感染者外周血中的变化及临床意义,收集35例患者[其中肝功能正常(liver function normal,LFN)者23例、肝功能异常(liver function abnormal,LFAN)者12例]和20例正常对照(normal control,NC)者外周血。FACS检测各组外周血中3种非传统淋巴细胞(iNKT细胞、γδT细胞、B1细胞)和T细胞、B细胞、NK细胞的相对含量、计算出各细胞的绝对数,并分析各类细胞相对含量与临床指标丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)水平的相关性。结果显示,与NC组比较,LFN组和LFAN组iNKT细胞的相对和绝对计数均显著降低(P<0.01,P<0.05;P<0.01,P<0.01);与HBV-LFN组比较,HBV-LFAN组γδT细胞的相对和绝对计数均显著升高(均P<0.05);与NC组比较,HBV-LFAN组T细胞相对计数显著升高(P<0.05),而NK细胞的相对和绝对计数均显著降低(均P<0.05)。相关性分析结果显示,慢性HBV感染者的外周血γδT细胞相对含量与ALT水平呈正相关(r=0.4534,P<0.01);NK细胞含量与ALT、AST水平呈负相关(r=-0.5283,P<0.01;r=-0.5187,P<0.01);T细胞含量与AST水平呈正相关(r=0.3950,P<0.05);B1细胞含量与AST水平呈负相关(r=-0.3937,P<0.05)。该研究提示,慢性HBV感染者外周血中包括非传统淋巴细胞在内的多种淋巴细胞亚群含量变化明显,且与患者肝功能受损情况相关。
The purpose of this study is to investigate the changes and clinical significance of three unconventional subsets of lymphocytes:invariant natural killer T cell(iNKT),γδT cell and B1 cell,as well as T cell,B cell and NK cell in peripheral blood of chronic HBV infected patients.To this end,a total of 23 HBV patients with normal liver function,12 HBV patients with abnormal liver function,and 20 normal controls(NC)were stratified to HBV-LFN(liver function normal)group,HBV-LFAN(liver function abnormal)group and NC group,respectively.The relative amount of iNKT cell,γδT cell,B1 cell,T cell,B cell and NK cell in peripheral blood was measured by FACS.Next,the absolute amount of these cells was calculated and the correlation between the relative amount of these lymphocyte subsets and clinical indexes including alanine aminotransferase(ALT)and aspartate aminotransferase(AST)was analyzed.The results showed that the relative and absolute counts of iNKT cell in peripheral blood of HBV-LFN group and HBV-LFAN group were significantly lower than those in NC group(P<0.01 and P<0.05 for HBV-LFN group;P<0.01 and P<0.01 for HBV-LFAN group).Compared to those of HBV-LFN group,the relative and absolute counts ofγδT cell were significantly higher in HBV-LFAN group(both P<0.05).The relative counts of T cell in HBV-LFAN group were significantly higher than those in NC group(P<0.05),while the relative and absolute counts of NK cell in HBV-LFAN group were significantly lower than those in NC group(both P<0.05).Additionally,in chronic HBV infected patients,there was a significant positive correlation between the relative counts ofγδT cell and ALT level(r=0.4534,P<0.01),a significant negative correlation between NK cell counts and ALT or AST levels(r=-0.5283,P<0.01;r=-0.5187,P<0.01,respectively),a significant positive correlation between T cell counts and AST level(r=0.3950,P<0.05)and a significantly negative correlation between B1 cell counts and AST level(r=-0.3937,P<0.05).In conclusion,the composition of peripheral blood lymphocyte subsets including unconventional lymphocytes changes significantly in chronic HBV infected patients,which correlates with compromised liver function.
作者
姚春艳
胡召锁
袁润林
陈正徐
YAO Chun-yan;HU Zhao-suo;YUAN Run-lin;CHEN Zheng-xu(Department of Laboratory,Hefei Hospital Affiliated to Anhui Medical University/The Second People's Hospital of Hefei,Hefei 230011,China)
出处
《现代免疫学》
CAS
北大核心
2023年第1期42-47,59,共7页
Current Immunology
基金
合肥市卫健委课题(Hwk2020yb004)。
