摘要
目的探讨大豆皂苷Bb经丝裂原细胞外信号调节激酶/细胞外信号调节激酶(MEK/ERK)信号通路对牙槽骨成骨细胞增殖、凋亡的影响。方法培养人牙槽骨成骨细胞,Dulbecco改良的Eagle培养基(DMEM)培养的细胞作为对照组(A组),以含400μg/mL雌二醇、400μg/mL和800μg/mL大豆皂苷Bb的DMEM培养的细胞分别作为雌二醇组(B组),大豆皂苷Bb低、高剂量组(C_(1)组和C2组)。采用MTT法测定细胞活力和计数细胞菌落数,采用流式细胞术测定细胞凋亡水平,采用逆转录实时荧光定量聚合酶链反应(qRT-PCR)法及蛋白印迹法测定细胞中MEK,ERK mRNA和蛋白表达水平。结果与A组比较,B组、C_(1)组、C2组光密度(OD)、细胞存活率、菌落数、MEK及ERK mRNA和蛋白表达水平均明显升高(P<0.05),且呈剂量依赖性;与B组比较,C_(1)组上述指标均明显降低(P<0.05),而C2组无明显差异(P>0.05)。与A组比较,B组、C_(1)组、C2组细胞凋亡率均明显降低(P<0.05),且呈剂量依赖性;与B组比较,C_(1)组细胞凋亡率明显升高(P<0.05),C2组细胞凋亡率无明显差异(P>0.05)。结论大豆皂苷Bb能促进牙槽骨成骨细胞增殖,抑制凋亡。其作用机制可能与大豆皂苷Bb促进牙槽骨成骨细胞MEK,ERK mRNA和蛋白表达,以及激活MEK/ERK信号通路传导有关。
Objective To investigate the effect of soyasaponin Bb on the proliferation and apoptosis of alveolar osteoblasts through the mitogen extracellular signal-regulated kinase/extracellular signal-regulated kinase(MEK/ERK)signal pathway.Methods The human alveolar osteoblasts cultured in the Dulbecco′s modified Eagle medium(DMEM)were used as the control group(group A),the human alveolar osteoblasts cultured in the DMEM containing 400μg/mL estradiol,400μg/mL soyasaponin Bb and 800μg/mL soyasaponin Bb were used as the estradiol group(group B),soyasaponin Bb low-and high-dose groups(groups C_(1)and C_(2))respectively.The cell viability and cell colony count were measured by the MTT method,the apoptosis level was measured by the flow cytometry,and the expression levels of MEK,ERK mRNA and protein were measured by the reverse transcription real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)and Western blot respectively.Results Compared with those in the group A,the optical density(OD),survival rate of cells,colony count,expression levels of MEK,ERK mRNA and protein in the groups B,C_(1)and C_(2)were significantly higher in a dose-dependent manner(P<0.05).Compared with those in the group B,the levels of the above indexes in the group C_(1)were significantly lower(P<0.05),while those in the group C_(2)were similar to those in the group B(P>0.05).Compared with that in the group A,the apoptosis rate in the groups B,C_(1)and C_(2)was significantly lower in a dose-dependent manner(P<0.05).Compared with that in the group B,the apoptosis rate in the group C_(1)was significantly higher(P<0.05),while that in the group C_(2)was similar to that in the group B(P>0.05).Conclusion Soyasaponin Bb can promote the proliferation of alveolar osteoblasts and inhibit their apoptosis.Its mechanism may be related to soyasaponin Bb promoting the expression of MEK,ERK mRNA and protein in alveolar osteoblasts and activating the MEK/ERK signaling pathway.
作者
管琴
刘姣
阳刘康
任伟伟
GUAN Qin;LIU Jiao;YANG Liukang;REN Weiwei(Dongfeng Stomatological Hospital Affiliated to Hubei University of Medicine,Shiyan,Hubei,China 442000;School of Basic Medicine,Hubei University of Medicine,Shiyan,Hubei,China 442000)
出处
《中国药业》
CAS
2023年第5期46-50,共5页
China Pharmaceuticals
基金
湖北省十堰市科学技术局引导性科研项目[19Y102]。
