摘要
目的评价桦褐孔菌药材质量,建立高效液相色谱(HPLC)法测定桦褐孔菌药材中4种成分含量,并比较不同部位4种成分含量的差异。方法采用HPLC法测定桦褐孔菌中栓菌酸、桦褐孔菌醇、麦角甾醇、羊毛甾醇含量,并对桦褐孔菌药材黑褐色外壳和内部黄色菌肉中4种成分含量进行比较。色谱柱为Agilent 5 TC-C 18(250 mm×4.6 mm,5μm),柱温30℃;以乙腈-水为流动相,梯度洗脱,流速为1.0 mL·min^(-1);检测波长为203,282 nm;进样量:20μL。采用SPSS23.0版统计软件对不同产地桦褐孔菌药材进行聚类分析,对内外两部位含量进行统计学分析。结果4种成分在各自浓度范围内线性关系良好(r≥0.9997);平均加样回收率分别为101.79%,102.96%,101.12%和101.37%,RSD分别为2.35%,2.25%,2.27%和2.13%(n=6);精密度、重复性、稳定性(24 h)实验RSD均<3.0%(n=6);23批桦褐孔菌药材中栓菌酸、桦褐孔菌醇、麦角甾醇、羊毛甾醇含量范围分别为0.3830~3.5234,0.7412~6.4903,0.1288~0.6997,0.6289~4.0533 mg·g^(-1);聚类分析可将23批样品分为3类。桦褐孔菌药材内部菌肉中麦角甾醇、羊毛甾醇含量高于黑褐色外壳,而栓菌酸、桦褐孔菌醇含量在桦褐孔菌内外两部位之间差异无统计学意义。结论所建立的含量测定方法重复性、稳定性、可操作性良好,可为桦褐孔菌药材的质量控制提供参考。
Objective To establish an HPLC method to determine four components of Inonotus obliquus,to compare the content differences of the four components in different parts,and evaluate the quality of Inonotus obliquus.Methods The contents of trametenolic acid,Inotodiol,ergosterol,and lanosterol of Inonotus obliquus were determined by the HPLC method,the contents of four components in the dark brown shell and yellow internal context of Inonotus obliquus were compared.The analysis was performed on an Agilent 5 TC-C 18 column(250 mm×4.6 mm,5μm),and the column temperature was 30℃.The mobile phase was acetonitrile-water at a flow rate of 1 mL·min^(-1)in a gradient elution manner.The detector wavelength was set at 203,208 nm.SPSS.23.0 software was used for cluster analysis of Inonotus obliquus from different habitats,statistical analysis was made on the content difference between the two parts.Results The linearity of the four components was good in the range of their mass concentration(r≥0.9997);the average recoveries were 101.79%,102.96%,101.12%and 101.37%;with RSDs of 2.35%,2.25%,2.27%and 2.13%(n=6);RSDs of precision,repeatability,and stability(24 h)tests were all less than 3.0%(n=6),respectively.The contents of trametenolic acid,inotodiol,ergosterol and lanosterol in 23 batches of Inotodiol obliquus were 0.3830-3.5234,0.7412-6.4903,0.1288-0.6997,0.6289-4.0533 mg·g^(-1),respectively.The 23 batches of samples could be divided into three categories by cluster analysis.The contents of ergosterol and lanosterol in the yellow internal context of Inonotus obliquus were higher than those in the dark brown shell,but there was no significant difference in the contents of trametenolic acid and Inonotol between the internal and external parts of Inonotus obliquus.Conclusion The content determination method has good repeatability,stability and operability,which can provide reference for the quality control of Inonotus obliquus.
作者
段雨晴
朱田密
陈树和
段雪云
王思萌
DUAN Yuqing;ZHU Tianmi;CHEN Shuhe;DUAN Xueyun;WANG Simeng(School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China;Department of Pharmay,Hubei Provincial Hospital of Traditional Chinese Medicine/Affiliated Hospital of Hubei University of Traditional Chinese Medicine Wuhan 430061,China;Hubei Institute of Traditional Chinese Medicine,Wuhan 430074,China)
出处
《医药导报》
CAS
北大核心
2023年第3期339-345,共7页
Herald of Medicine
基金
湖北省科技重大专项重点研发计划项目(2020ACA007)
湖北省自然科学基金创新发展联合基金项目(2022FD162)。
关键词
桦褐孔菌
栓菌酸
桦褐孔菌醇
麦角甾醇
羊毛甾醇
含量测定
高效液相色谱法
Inonotus obliquus
Trametenolic acid
Inotodiol
Ergosterol
Lanosterol
Content determination
High performance liquid chromatography(HPLC)
