摘要
基于邻苯二甲酸酯(phthalic acid ester,PAEs)对双金属原子Cu,Fe-N-C纳米酶模拟氧化酶活性的调节,建立简单、灵敏测定发酵食品中PAEs的荧光探针方法。PAEs吸附于Cu,Fe-N-C表面后,会加速Cu,Fe-N-C催化氧化底物邻苯二胺,使生成的2,3-二氨基吩嗪产物荧光强度增加,PAEs在1.25~50μg/L范围内呈良好的线性关系。将该研究方法用于PAEs含量的测定,其检出限可达到0.76μg/L,实际样品的加标回收率在88.0%~104.6%范围内,满足GB 5009.271—2016中的检测要求。该方法具有简便、稳定、快速等特点,其检出限也低于国家规定的食品中PAEs含量的限值。
A simple and sensitive fluorescent probe for the determination of phalic acid ester(PAEs) in fermented foods was developed based on the fact that the oxidase-like activity of Cu,Fe-N-C nanozyme can be regulated by PAEs. After PAEs were adsorbed on the surface of Cu,Fe-N-C, the catalytic oxidation of the substrate o-phenylenediamine(OPD)was accelerated, thus increasing the fluorescence intensity of the generated 2,3-diaminophenazine(DAP). The calibration curve for PAEs showed a good linear relationship in the concentration range of 1.25–50 μg/L. The detection limit of the proposed method was 0.76 μg/L. The recoveries of spiked samples ranged from 88.0% to 104.6%, which meet the detection requirements of the national standard GB 5009.271-2016. This method is simple, stable and rapid, and its detection limit is lower than the national limit for PAEs content in foods.
作者
李宏
史巧
陈骏飞
王馨蕊
汤回花
李秋兰
杨德志
杨亚玲
LI Hong;SHI Qiao;CHEN Junfei;WANG Xinrui;TANG Huihua;LI Qiulan;YANG Dezhi;YANG Yaling(Institute of Agro-products Processing,Yunnan Academy of Agricultural Sciences,Kunming 650221,China;Faculty of Life Science and Technology,Kunming University of Technology,Kunming 650500,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第4期306-312,共7页
Food Science
基金
云南省重大科技专项(202002AE320006)。
关键词
邻苯二甲酸酯
纳米酶
氧化酶活性
荧光探针
phthalic acid esters
nanozyme
oxidase-like activity
fluorescent probe
