摘要
该实验建立并优化了一种顶空固相微萃取-气相色谱法(HS-SPME-GC)检测不同果酒中甲醇和杂醇的研究方法,利用该方法对葡萄酒、山楂酒、猕猴桃酒3种果酒中的甲醇和杂醇含量进行测定,并跟踪测定3种果酒陈酿过程中8种组分的含量变化。结果表明,样品的萃取选择PDMS固相微萃取头,50℃顶空萃取40 min,8种组分采用CP-WAXMS色谱柱(60 m×250μm×0.25μm)分离。在此条件下,各化合物在0.01~50 mg/mL范围内线性关系良好(R>0.999),检出限为0.001~0.005 mg/mL,定量限为0.003~0.015 mg/mL,加标回收率为88.3%~96.7%,精密度试验结果相对标准偏差(RSD)为2.7%~5.1%。随着陈酿时间的延长,葡萄酒中甲醇含量下降最快,杂醇总量呈增长趋势,而山楂酒、猕猴桃酒的杂醇总量则有不同程度的下降。该方法具有前处理简单、灵敏度高和检测速度快的优点,适用于果酒中甲醇及杂醇含量分析,为其产品质量的评判提供参考。
A method for the determination of methanol and fusel in different fruit wines by headspace solid phase microextraction-gas chromatography(HS-SPME-GC)was established and optimized.The contents of methanol and fusel in grape wine,hawthorn wine and kiwi wine were determined by the method,and the content changes of 8 components in 3 kinds of fruit wine were tracked and measured during the aging process.The results showed that PDMS solid phase microextraction head was selected for sample extraction,and the headspace extraction was performed at 50℃for 40 min.The 8 components were separated by CP-WAXMS(60 m×250μm×0.25μm)chromatographic column.Under these conditions,the linear relationship(R>0.999)of each component was good within the range of 0.01-50 mg/ml,the detection limit was 0.001-0.005 mg/ml,the quantitation limit was 0.003-0.015 mg/ml,the recovery rate was 88.3%-96.7%,and the relative standard deviation(RSD)of precision tests results was 2.7%-5.1%.With the extension of aging time,the methanol content in grape wine decreased the fastest,and the total amount of fusel showed an increasing trend,while the total amount of fusel in hawthorn wine and kiwi wine decreased to varying degrees.The method had the advantages of simple pretreatment,high sensitivity and fast detection speed.It was suitable for the content analysis of methanol and fusel in fruit wine,and provided a reference for the judgement of product quality.
作者
仲惟
李涛
ZHONG Wei;LI Tao(College of Food Landscape Architecture,Sanmenxia Vocational and Technical College,Sanmenxia 472000,China;College of Applied Engineering,Henan University of Science and Technology,Sanmenxia 472000,China)
出处
《中国酿造》
CAS
北大核心
2023年第2期245-250,共6页
China Brewing
基金
三门峡市科技发展计划项目(2019070207)。
关键词
顶空固相微萃取
毛细管气相色谱
果酒
甲醇
杂醇
陈酿
headspace solid phase microextraction
gas chromatography
fruit wine
methanol
fusel
aging
