摘要
盐碱地微生物长期在盐碱环境中共存与进化,具有独特适应性和生物学特性,成为微生物改良盐碱地的首选。从吉林省松原市典型苏打盐碱地土壤中分离到1株耐盐碱真菌菌株SYAT-1,通过形态特征观察和ITS-rDNA序列分析对该菌株进行鉴定,并对该菌株不同pH生长特性、耐盐性、拮抗植物病原真菌活性和潜在致病性进行研究。结果表明,该菌株鉴定为土曲霉(Aspergillus terreus)。菌株SYAT-1在碱性(pH 7.0-12.0)培养基条件下均可生长,其生长最适pH范围为7.0-9.0。在0-17.5 mg/mL NaCl和0-4 mg/mL NaHCO_(3)的PDA培养基上均能生长。拮抗植物病原真菌测定结果显示,菌株SYAT-1对供试植物病原真菌均有一定的抑制效果。潜在致病性测试结果表明,菌株SYAT-1对供试植物无潜在的致病性。土曲霉菌株SYAT-1属于耐高盐碱特性的真菌,并具有抑制植物病原真菌的特性,该结果为利用微生物改良盐碱土和植物病害生物防治提供新的菌株资源和理论支撑。
Microorganisms co-exist and evolve in saline environment for a long time and have unique adaptability and biological properties,which make them the first choice in the microbial improvement of saline land.A saline-alkali tolerant fungal strain SYAT-1 was isolated from a typical soda saline soil in Songyuan city,Jilin province.It was identified by morphological characteristics observation and ITSrDNA sequence analysis.The growth characteristics at different pH,salt tolerance,antagonistic activity against phytopathogenic fungi and potential pathogenicity of the strain were investigated.The results showed that it was identified as Aspergillus terreus and grew under alkaline(pH=7.0-12.0)medium conditions,with an optimal pH range of 7.0-9.0.It grew well on both 0-17.5 mg/mL NaCl PDA medium and 0-4mg/mL NaHCO_(3) PDA medium.Antagonistic phytopathogenic fungi assay results revealed that strain SYAT-1 presented some inhibitory effect on the test phytopathogenic fungi.Potential pathogenicity test results demonstrated that strain SYAT-1 had no potential pathogenicity to the test plants.In sum,A.terreus strain SYAT-1 belongs to a fungus with high salt tolerance characteristics and suppressing phytopathogenic fungi.This study provides new strain resources and theoretical support for the use of microorganisms to improve saline soils and biological control of plant diseases.
作者
王凤婷
王岩
孙颖
崔文婧
乔凯彬
潘洪玉
刘金亮
WANG Feng-ting;WANG Yan;SUN Ying;CUI Wen-jing;QIAO Kai-bin;PAN Hong-yu;LIU Jin-liang(College of Plant Sciences,Jilin University,Changchun 130062)
出处
《生物技术通报》
CAS
CSCD
北大核心
2023年第2期203-210,共8页
Biotechnology Bulletin
基金
吉林省重点研发计划项目(20210202131NC)
国家重点研发计划政府间国际合作专项(2019YFE0114200)
国家重点研发计划项目(2016YFC0501200)
长春市重点研发计划项目(21ZGN13)。
关键词
耐盐碱
土曲霉
分离鉴定
病原真菌
抑制
saline-alkali tolerance
Aspergillus terreus
isolation and identification
pathogenic fungus
inhibition
