摘要
分析大孔树脂与人参皂苷之间的吸附行为,优化大孔树脂分离人参皂苷的条件。选用6种大孔树脂(D101、HPD-100、AB-8、NKA-9、ADS-7、DM130),以二醇型人参皂苷(Rg_(1)、Re、Rg_(2))和三醇型人参皂苷(Rc、Rb_(1)、Rd)的含量为评判指标,进行吸附率、解吸率与吸附容量比较,发现纯化人参皂苷的最适大孔树脂为HPD-100。然后对其静态吸附时间、吸附温度、吸附初始浓度与动态加载流速、加载量、洗脱溶剂等进行考察,筛选最佳纯化工艺。二醇型人参皂苷与三醇型人参皂苷在HPD-100大孔树脂上静态吸附量为109 mg/g,吸附率分别为99.93%与56%,解吸率分别为96.4%与98.5%,静态最佳吸附时间为190 min,吸附初始浓度为32 mg/mL,温度为35℃,动态加载流速为4 BV/h(每小时4个柱体积),加载量8 BV(柱体积),洗脱剂为40%和60%乙醇。最终三醇型皂苷的纯化率为66%,二醇型皂苷的纯化率为52%。表明HPD-100D大孔树脂可以用于二醇型人参皂苷和三醇型人参皂苷的分离纯化。
The adsorption behavior between macroporous resin and ginsenosides were analyzed and the conditions for the separation of ginsenosides from macroporous resin were optimized.The adsorption rate,desorption rate and adsorption capacity of six macroporous resins(D101,HPD-100,AB-8,NKA-9,ADS-7,DM130)were compared with the content of diol-type ginsenosides(Rg_(1),Re,Rg_(2))and triol-type ginsenosides(Rc,Rb_(1),Rd)as the evaluation index.To compare the adsorption rate,desorption rate and adsorption capacity,the most suitable macroporous resin for the purification of ginsenosides was found to be HPD-100.After that,the static adsorption time,adsorption temperature,initial concentration of adsorption and dynamic loading flow rate,loading volume and elution solvent were investigated to screen the best purification process.Statically,the adsorption of diol and triol ginsenosides on HPD-100 macroporous resin was 109 mg/g with 99.93%and 56%adsorption and desorption rates of 96.4%and 98.5%,respectively,with a static optimum adsorption time of 190 min,an initial concentration of 32 mg/g at 35℃,a dynamic loading flow rate of 4 BV/h(4 column volumes per hour),loading volume of 8 BV(column volume),eluent of 40% and 60% ethanol.The purification rates were 66%for triol ginsenosides and 52%for diol ginsenosides.HPD-100D macroporous resin could be used for the isolation and purification of diol and triol ginsenosides.
作者
王斌
赵升铭
袁松竹
王义
张美萍
雷军
陈屏
WANG Bin;ZHAO Sheng-ming;YUAN Song-zhu;WANG Yi;ZHANG Mei-ping;LEI Jun;CHEN Ping(College of Life Science,Jilin Agricultural University,Changchun 130118,China;Jilin Engineering Research Center for Ginseng Genetic Resources Development and Utilization,Changchun 130118,China)
出处
《化学试剂》
CAS
北大核心
2023年第3期67-73,共7页
Chemical Reagents
基金
吉林省教育厅“十三五”科学技术项目(JTKF20200362KJ)
吉林省科技发展计划项目(20190201171JC)。
关键词
大孔树脂
人参皂苷
分离纯化
静态吸附
动态吸附
macroporous resin
ginsenoside
separation and purification
static adsorption
dynamic adsorption
