摘要
为获得鹿茸草的全长转录组信息,挖掘鹿茸草次生代谢化合物生物合成途径相关酶的基因,该文基于单分子测序技术,利用Pacbio高通量测序平台,对鹿茸草进行全长转录组测序,共获得48005条去冗余的高质量转录本,与NR、Swiss-Prot、GO、KEGG等8个数据库进行BLAST比对,共有45362个转录本被成功注释,注释率为94.50%.其中有389条转录本被注释到KEGG的10条标准次生代谢生物合成通路中.对转录组数据进一步分析发现:参与鹿茸草苯丙素类生物合成的转录本有194条,参与生物碱类生物合成的转录本有115条,参与类黄酮化合物生物合成的转录本有23条,参与其他次生代谢产物的转录本有57条,参与次生代谢后氧化与糖基化修饰的转录本有204条.鹿茸草全长转录组的获得极大地丰富了鹿茸草的遗传信息,初步揭示了参与鹿茸草次生代谢产物合成相关的基因通路,为深入研究鹿茸草次生代谢产物合成途径关键酶的功能及其调控机制奠定了基础.
To obtain the full-length transcriptome sequence database and mine the genes of enzymes related to the secondary metabolic compounds′biosynthetic pathway in Monochasma savatieri,based on Single Molecule Real Time(SMRT)technology,the full-length transcriptome is sequenced using Pacbio high-throughput sequencing platform.A total of 48005 high-quality transcripts are obtained.45362 transcripts are successfully annotated by BLAST search against NR,Swiss-Prot,GO,KEGG and other four public sequence databases,with an annotation rate of 94.50%.Among them,389 transcripts are annotated to the 10 standard secondary metabolic biosynthetic pathways of KEGG.Further analysis reveals that a total of 194 transcripts are involved in phenylpropanoid biosynthesis,115 transcripts are related to alkaloid biosynthesis,23 transcripts might participate in flavonoid biosynthesis,and 57 transcripts are involved in other secondary metabolites.Additionally,204 transcripts relate to secondary metabolic oxidation and glycosylation post-modifications.The obtained data of this study greatly enriches the genetic information of Monochasma savatieri and preliminarily reveals the genes involved in the synthesis of secondary metabolites,which lays a foundation for further studies on function and regulatory mechanisms of key genes involved in thesynthesis of secondary metabolites in Monochasma savatieri.
作者
杨晚铃
白芷懿
邹明珠
王欣茹
谢建坤
张帆涛
YANG Wanling;BAI Zhiyi;ZOU Mingzhu;WANG Xinru;XIE Jiankun;ZHANG Fantao(College of Life Sciences,Jiangxi Normal University,Nanchang Jiangxi 330022,China)
出处
《江西师范大学学报（自然科学版）》
CAS
北大核心
2023年第1期99-110,共12页
Journal of Jiangxi Normal University(Natural Science Edition)
基金
国家自然科学基金(31960370)
江西省自然科学基金(20202ACB205002)资助项目.
关键词
鹿茸草
全长转录组
次生代谢
生物合成
遗传资源
Monochasma savatieri Franch.ex Maxim
full-length transcriptome
secondary metabolism
biosynthesis
genetic resource
