摘要
利用生物信息学方法在线分析生长抑素Ⅱ型受体SSTR2的理化性质、信号肽、跨膜结构、分泌蛋白类型、亚细胞定位、磷酸化位点修饰、空间结构及蛋白互作网络等,并通过MEGA5.0软件建立SSTR2蛋白的系统进化树。结果显示,该基因编码369个氨基酸,分子式为C_(1898)H_(2984)N_(470)O_(513)S_(23),相对分子质量为41332.79,等电点理论值为9.15,不稳定系数为37.16。SSTR2是一种碱性稳定亲水蛋白,无信号肽,存在7个跨膜区,作用于质膜结构;二级结构主要为α-螺旋,属7tmGPCRs超家族,且含有1个7tmA_SSTR2结构域;与SSTR2相互作用的蛋白质包括CORT、SST、NPY、GHRL、GNAI1、GNAI2、GNAI3、SHANK1、HIVEP2。SSTR2基因及其编码蛋白在进化上高度保守,可能参与G蛋白偶联受体信号通路,进而发挥其作用。本研究通过对SSTR2蛋白的理化性质及生物学功能进行分析,为深入研究该蛋白在疾病发生中的机制以及为靶向治疗神经内分泌肿瘤等肿瘤药物研发提供理论依据。
The physical and chemical properties,signal peptides,transmembrane structure,secretory protein types,subcellular localization,phosphorylation site modification,secondary structure and protein interaction of the human somatostatin typeⅡreceptor(SSTR2)were analyzed by online bioinformatics software such as NCBI,ExPASy,SignalP,PSORTII,SOPMA,STRING,SWISS-MODEL,and the phylogenetic tree of SSTR2 protein was established by MegAlign software.The results showed that,the protein encoded by the gene contains 369 amino acids,the molecular formula was C _(1898) H _(2984) N _(470 )O _(513) S _(23),the relative molecular weight was 41332.79,the theoretical value of isoelectric point was 9.15 and the instability coefficient was 37.16.SSTR2 was a stable alkaline hydrophilic protein with no signal peptide and 7 transmembrane regions,which acts on the plasma membrane structure.The secondary structure was mainlyα-Helix belonging to 7tmGPCRs superfamily and containing one 7tmA_SSTR2 domain;proteins interacting with SSTR2 include CORT,SST,NPY,GHRL,GNAI1,GNAI2,GNAI3,SHANK1,HIVEP2.SSTR2 gene and its encoding protein were highly conserved in evolution,and may be involved in the G protein coupled receptor signal pathway and play its role.In this study,the physical and chemical properties and biological functions of SSTR2 gene were analyzed to provide theoretical basis for the in-depth study of the mechanism of SSTR2 protein in the occurrence of diseases and the research and development of targets therapies for neuroendocrine tumors and other cancer drugs.
作者
张丽萌
李闰婷
聂晓宁
李玉华
李林
李亚蒙
陈龙欣
王林青
ZHANG Limeng;LI Runting;NIE Xiaoning;LI Yuhua;LI Lin;LI Yameng;CHEN Longxin;WANG Linqing(Laboratory of Molecular Biology,Zhengzhou Normal University,Zhengzhou Henan 450044,China;School of Life Science,Zhengzhou Normal University,Zhengzhou Henan 450044,China)
出处
《广西师范大学学报（自然科学版）》
CAS
北大核心
2023年第1期164-173,共10页
Journal of Guangxi Normal University:Natural Science Edition
基金
国家自然科学基金(32071447)
河南省高等学校重点科研项目资助计划(22A180031)
河南省高校科技创新人才支持计划(22HASTIT041)
河南省科技厅科技发展计划(科技攻关)项目(212102310901)
郑州师范学院大学生科研创新基金(2021013)
德阳市科技局社会领域重点研发项目(2021SZ018)。
