摘要
目的:研究莪术醇对血管内皮生长因子(VEGF)诱导的新生血管生成的作用及机制。方法:体外培养人脐静脉血管内皮细胞,用50ng/mL VEGF和不同浓度莪术醇进行分组处理,采用CCK-8和EdU实验检测细胞增殖,Transwell实验分析细胞迁移能力,管腔形成实验分析内皮细胞血管生成能力,Western blot检测Akt/mTORC1通路变化。结果:CCK-8实验结果显示,400、800μmol/L莪术醇+VEGF组细胞OD450值明显低于VEGF组(均P<0.01)。EdU结果显示,400μmol/L莪术醇+VEGF组细胞增殖率明显低于VEGF组(P<0.001)。Transwell实验和管腔形成实验结果显示,与VEGF组比较,400μmol/L莪术醇+VEGF组迁移细胞数减少,管腔形成的分支数量和分支长度下降(均P<0.001)。Western blot结果显示,莪术醇可显著减少细胞中Akt/mTORC1下游靶点p-Akt和p-S6的表达。结论:莪术醇可抑制VEGF诱导的血管内皮细胞的增殖、迁移和管腔形成,具有强的抑制血管生成的作用,可进一步用于眼底新生血管的治疗研究。
·AIM:To study the role and mechanism of curcumol in neovascularization induced by vascular endothelial growth factor(VEGF).·METHODS:Human umbilical vein endothelial cells were cultured in vitro and treated with 50ng/mL VEGF and curcumol at different concentrations.Cell proliferation was detected by CCK-8 and EdU assay,the migration ability of cells was analyzed by Transwell assay,the angiogenesis ability of endothelial cells was analyzed by tube formation assay,and the change of Akt/mTORC1 signal pathway was detected by Western blot.·RESULTS:CCK-8 results showed that the OD450 value of cells in 400 and 800 μmol/L curcumol+VEGF group was significantly lower than that in VEGF group(all P<0.01).EdU results showed that the rate of cell proliferation in 400 μmol/L curcumol+VEGF group was significantly lower than that in VEGF group(P<0.001).Transwell assay and the formation assay results showed that the number of migratory cells in 400 μmol/L curcumol+VEGF group was decreased,and the number and length of tube branches were also reduced compared with VEGF group(all P<0.001).Western blot results showed that curcumol significantly inhibited the expression of p-Akt and p-S6,which were downstream targets of Akt/mTORC1 pathway in cells.·CONCLUSION:Curcumol can inhibit VEGF-induced cell proliferation,migration and tube formation of vein endothelial cells,and has a strong inhibitory effect on angiogenesis,which can be further studied in the treatment of ocular fundus neovascularization.
作者
郝雨檬
王彩霞
马景学
李雪景
尚庆丽
Yu-Meng Hao;Cai-Xia Wang;Jing-Xue Ma;Xue-Jing Li;Qing-Li Shang(Department of Ophthalmology,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei Province,China;Department of Ophthalmology,No.980 Hospital of Joint Logistic Support Force of People’s Liberation Army of China,Shijiazhuang 050051,Hebei Province,China)
出处
《国际眼科杂志》
CAS
北大核心
2023年第3期379-384,共6页
International Eye Science
基金
河北省自然科学基金项目(No.H2021206009,H2022206287)。