摘要
目的 对枣庄市1起学校乙型Victoria系(BV系)流感聚集性疫情的病原学和遗传特征进行分析,为BV系流感的防控提供参考依据。方法 收集13例患者的咽拭子标本进行核酸检测和病毒分离,对毒株进行全基因组测序,利用生物信息学软件构建进化树并分析分子进化特征。结果 引起本次学校聚集性感染的病原体为BV系流感病毒,核酸阳性率为76.92%(10/13),分离毒株5株。与疫苗株B/Washington/02/2019相比,核苷酸同源性为98.4%~99.6%。血凝素(HA)进化树中5株BV系毒株与疫苗株B/Washington/02/2019共同归属于1A分支。HA蛋白9个氨基酸位点发生置换,其中6个位点发生在抗原决定簇,未发现神经氨酸酶(NA)抑制剂耐药位点和酸性聚合酶(PA)抑制剂耐药位点发生变异。HA均发生N197D置换,M1均发生T15I置换,多个蛋白的磷酸化位点发生改变。结论 引起枣庄市学校聚集性疫情的病原体是BV系流感病毒,应加强学校疫情监测并督促防控措施落实。
Objective This paper aims to analyze the epidemiological and genetic characteristics of an influenza B Victoria lineage(BV lineage) cluster outbreak in a school in Zaozhuang City, so as to provide reference basis for the prevention and control of BV lineage influenza. Methods Pharyngeal swab samples of 13 patients were collected for nucleic acid detection and virus isolation. The whole genome of the virus strains was sequenced. Bioinformatics software was used to construct evolutionary tree and analyze molecular evolutionary characteristics. Results The pathogen causing the cluster infection in this school was BV influenza virus, the positive rate of nucleic acid was 76.92%(10/13), and 5 strains were isolated. Compared with vaccine strain B/Washington/02/2019, the nucleotide homology was between 98.4%-99.6%. Five BV strains and vaccine strain B/Washington/02/2019 belong to the 1A branch of the HA evolutionary tree. 9 amino acid sites of HA protein were replaced, 6 of which were in the epitope. No variation was found in the NA inhibitor resistance sites(neuraminidase) and acid polymerase(PA) inhibitor resistance sites. N197D replacement was observed in HA, T15I replacement was observed in M1, and phosphorylation sites of multiple proteins were changed. Conclusion The pathogen causing the cluster epidemic in a school of Zaozhuang City is the BV influenza virus. It is necessary to strengthen the epidemic monitoring in schools and urge the implementation of prevention and control measures.
作者
张涛
张亮
谢运清
龙琳
ZHANG Tao;ZHANG Liang;XIE Yun-qing;LONG Lin(Zaozhuang Municipal Center for Disease Control and Prevention,Shandong 277000,China)
出处
《中国卫生检验杂志》
CAS
2023年第3期316-319,共4页
Chinese Journal of Health Laboratory Technology
基金
枣庄市科技发展计划项目(2021NS57)。
关键词
BV系流感病毒
血凝素
全基因组分析
Influenza virus of the BV lineage
Hemagglutinin
Genome-wide analysis
