摘要
目的比较载玻片-原位培养法(以下简称原位法)和胰酶消化法在羊水细胞染色体检查中的应用价值。方法选取2020年7—12月来该院进行产前诊断的1105例孕妇的羊水标本,分别采用原位法和胰酶消化法进行培养、收获及制片,成功制片后用GSL-120扫描、捕获核型,对两种方法的羊水细胞培养成功率、培养时间、分裂相、核型结果等进行比较分析。结果1105例羊水标本同时采用原位法、胰酶消化法两种方法进行培养,这两种方法的培养成功率的差异无统计学意义(χ^(2)=0.33,P>0.05)。原位法和胰酶消化法羊水需求量分别为5、10 mL,培养基用量分别为7、8 mL。与胰酶消化法相比,原位法羊水细胞培养时间短,分裂相及优质分裂相数目多,差异均有统计学意义(Z=-31.83,P<0.001;Z=-2.937,P=0.003;Z=-2.943,P=0.003)。两种方法的羊水染色体核型分析均检出972例正常核型、128例异常核型,其中染色体数目异常真嵌合7例,染色体核型结果符合率为100%。结论与胰酶消化法相比,原位法具有培养时间短、培养基用量少、羊水需求量少、操作步骤简单、优质分裂相多,以及可以有效诊断真假嵌合等优点,但胰酶消化法相较于原位法具有制片可重复性相对较高且耗材相对经济的优势,结合原位法和胰酶消化法的优缺点,羊水细胞培养可以采用原位法为主、胰酶消化法为辅的培养模式,提高产前诊断的效率、准确率与成功率。
Objective To compare the application value of in situ slide culture(in situ culture)and trypsin digestion for chromosome examinations in prenatal diagnosis.Methods From July to December 2020,the amniotic fluid samples of 1105 pregnant women who came to a hospital for prenatal diagnosis were separately cultured,harvested and prepared in situ slide culture and trypsin digestion.After successful preparation,the GSL-120 scanner was used to scan and capture karyotypes.The cultural success rate,culture time,division phase,and karyotype results of the two methods were respectively compared and analyzed.Results 1105 cases of amniotic fluid specimens in situ culture and trypsin digestion at the same time,the cultural success rate of the two methods has no statistically significant difference(P>0.05).The amniotic fluid requirements in situ culture and trypsin digestion were 5 and 10 mL,and the medium dosage were 7 and 8 mL,respectively.Compared with trypsin digestion,amniotic fluid cell culture time was shorter,the number of mitotic phases and high-quality mitotic phases was more in situ culture,the differences were statistically significant(Z=-31.83,P<0.001;Z=-2.937,P=0.003;Z=-2.943,P=0.003).972 cases of normal karyotype and 128 cases of abnormal karyotype were all detected by both methods for chromosome karyotype analysis of amniotic fluid,which included 7 cases of abnormal chromosome number and true chimerism.The coincidence rate of chromosome karyotype results was 100%.Conclusion Compared with the trypsin digestion,the in situ culture has the following advantages,including short culture time,less medium consumption,less amniotic fluid demand,simple operation steps,more high-quality split phases,and high efficiency in diagnosing true and false chimerism.However,compared with in situ culture,the trypsin digestion has the advantages of relatively high repeatability and economic consumables.Combined with the advantages and disadvantages of in situ culture and trypsin digestion,in order to improve the efficiency,accuracy and success rate of prenatal diagnosis,amniotic fluid cell culture can be mainly cultured with in situ culture and supplemented with trypsin digestion.
作者
候舒文
陈薇
严雅兰
刘慧
袁静
HOU Shuwen;CHEN Wei;YAN Yalan;LIU Hui;YUAN Jing(Prenatal Diagnosis Center of Obstetrics and Gynecology,the First Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230022,China)
出处
《国际检验医学杂志》
CAS
2023年第5期526-530,共5页
International Journal of Laboratory Medicine
基金
国家重点研发计划重点专项(2021YFC1005303)。
关键词
羊水细胞
染色体
原位培养法
胰酶消化法
产前诊断
amniotic fluid cell
chromosome
in situ culture
trypsin digestion
prenatal diagnosis
