摘要
目的 基于生物信息学及体外药效研究,分析复方中药治疗肥胖的作用机制及疗效,为常用中药的创新应用提供依据。方法 运用TCMSP数据库获取中药复方制剂的活性成分及靶点,通过GEO数据库筛选差异基因芯片数据,绘制火山图。通过Cytoscape3.8.0构建活性成分与肥胖的差异基因靶点图,创建蛋白互作网络,进行GO、KEGG通路富集分析。最后采用MTT及Annexin V-FTIC/PI流式细胞术验证细胞生存及促凋亡效果。结果 获得中药复方制剂治疗肥胖的活性成分共204个,主要成分为槲皮素、山奈酚、木犀草素、黄芩素等;肥胖相关差异基因28个,对差异基因进行PPI拓扑网络构建,GO、KEGG通路涉及与胰岛素、激素、脂质及糖代谢相关的通路。MTT证实药物对3T3-L1小鼠前脂肪细胞有显著的浓度-时间依赖关系,72 h的IC50值为2.395 mg/mL;并可诱导前脂肪细胞的早期凋亡。结论 该研究提供了复方中药治疗肥胖的重要分子靶标、通路及作用机制,并进行体外药效学研究,为中药的临床应用提供了依据。
Objective To analyze the mechanism and efficacy of traditional Chinese medicine(TCM)compound preparation in treating obesity based on bioinformatics and efficacy study in vitro and provide the basis for the innovative application of commonly used TCM.Methods The active components and targets of TCM compound preparations were obtained by TCMSP. We screened the differential gene chip data by the GEO database to map the volcano. Cytoscape3.8.0 was used to construct the differential gene target map of TCM active ingredients and obesity,create a protein interaction network,and conduct an enrichment analysis of the GO and KEGG pathways. Finally,MTT and Annexin V-FTIC/PI flow cytometry were used to verify cell survival and apoptotic effect. Results A total of 204 active ingredients were obtained in TCM compound preparation for treating obesity,and the main components were quercetin,kaempferol,luteolin,baicalein,etc. There were 28 obesityrelated differential genes,and the PPI topological network of the differential genes was constructed. The GO and KEGG pathway was involved in the pathways related to insulin,hormone,lipid,and glucose metabolism. MTT confirmed that the drug had a significant concentration-time dependence on the preadipocytes of 3T3-L1 mice,and the IC50 value at 72 h was 2. 395 mg/mL. Moreover,it can induce the early apoptosis of preadipocytes.Conclusion This study provides important molecular targets,pathways,and mechanisms of action of TCM compound preparations in treating obesity through pharmacodynamics study in vitro,providing a basis for the clinical application of TCM.
作者
杨光
施璐
YANG Guang;SHI Lu(School of Medicine,Jianghan University,Wuhan 430056,Hubei,China)
出处
《江汉大学学报（自然科学版）》
2023年第1期52-63,共12页
Journal of Jianghan University：Natural Science Edition
