摘要
为了解CRISPR技术领域的研究现状,以中国知网(CNKI)中文数据库为数据来源筛选出的8031篇文献为研究对象,运用可视化软件CiteSpace.5.8 R3和Excel 2019分析CRISPR技术研究文献的年发文量、发文机构、基金、期刊、关键词等。在CNKI中文数据库中,CRISPR技术的年发文量在2014-2021年期间呈上升趋势,在2019年达到峰值。综合类院校、农业类院校和农科院是CRISPR技术研究的主要机构。发文量最多的作者是郑州大学段广才和湖北省农业科学院畜牧兽医研究所的毕延震,均为19篇。国家自然科学基金项目支持发表的论文数量最多,为2107篇,占比26.24%。高频关键词表明,当前农业领域CRISPR技术在水稻上的应用最为广泛。关键词中心性大小表明基因编辑、基因敲除、水稻等是当前国内CRISPR技术领域研究热点。关键词突现分析表明编辑效率、盐胁迫、活性氧、马铃薯、生长发育、过表达可能是该领域未来的研究热点。该论文从宏观上了解掌握CRISPR技术,为我国农业生物育种技术研究提供理论支撑。
In order to understand the research status in the field of CRISPR technology,taking 8031 documents screened from the Chinese Database of China National Knowledge Network(CNKI)as the data source,and using visualization software CiteSpace 5.8 R3 and Excel 2019,we analyzed the annual volume of CRISPR technical research literature,issuing institutions,funds,journals,keywords,etc.In CNKI Chinese database,the annual number of documents about CRISPR technology showed an upward trend from 2014 to 2021 and reached the peak in 2019.Comprehensive universities and colleges,agricultural universities and colleges and academies of agricultural sciences were the main institutions of CRISPR technology research.The authors with the largest number of published papers were Duan Guangcai of Zhengzhou University and Bi Yanzhen of the Institute of Animal Husbandry and Veterinary Medicine of Hubei Academy of Agricultural Sciences,both of them published 19 papers during 2000-2021.The number of papers supported by NSFC(National Natural Science Foundation of China)projects was the largest(2107),accounting for 26.24%.High frequency keywords showed that CRISPR technology was most widely used in rice in agricultural field.Keyword centrality indicated that gene editing,gene knockout and rice were the research hotspots in the field of CRISPR technology in China.Keyword emergence analysis showed that editing efficiency,salt stress,reactive oxygen species,potato,growth and development and overexpression might be the research hotspots in this field in the future.This paper understands and grasps CRISPR technology from a macro perspective,which provides theoretical support for the research of agricultural biological breeding technology in China.
作者
杨海峰
李梦荷
王雅丽
董彦琪
顾国兵
王泽
孙玉镯
申思洋
张栩
YANG Haifeng;LI Menghe;WANG Yali;DONG Yanqi;GU Guobing;WANG Ze;SUN Yuzhuo;SHEN Siyang;ZHANG Xu(Xinxiang Academy of Agricultural Sciences,Xinxiang,Henan 453000;Henan Women’s Vocational College,Zhengzhou 450000)
出处
《中国农学通报》
2023年第6期41-51,共11页
Chinese Agricultural Science Bulletin
基金
河南省软科学研究计划项目“河南省农业生物育种技术发展趋势与产业化途径研究”(222400410300)
新乡市政府招标课题“农业生物育种研发现状与产业化方向研究”(B21015)。
