摘要
利用气相色谱串联质谱法、棒状薄层色谱法、核磁共振波谱法和液相色谱法分析了不同部位高白鲑鱼油的化学成分,结果表明,肝脏中富含多不饱和脂肪酸(占总脂肪酸30.85%),尤其是二十碳五烯酸(EPA)(占总脂肪酸10.20%)和二十二碳六烯酸(DHA)(占总脂肪酸6.99%),明显高于脂肪和内脏组织中的多不饱和脂肪酸(20.38%和22.64%)、EPA(6.11%和6.89%)和DHA(2.75%和3.05%)。肝脏中生育酚质量分数为546.30μg/g,明显高于脂肪(127.25μg/g)和内脏组织(157.43μg/g);脂肪中生育三烯酚质量分数为61.18μg/g,明显高于肝脏(44.44μg/g)和内脏组织(49.51μg/g)。利用电子顺磁共振波谱法测定高白鲑鱼油的抗氧化性,结果表明肝脏中的鱼油具有显著的DPPH清除活性,EC 50为9.22 mg/mL。利用酶联免疫分析法测定鱼油的抗炎活性,结果表明鱼油可通过抑制NO、IL-1β、IL-6和TNF-α,促进IL-10和IL-13的分泌显示抗炎作用。高白鲑鱼油功能性脂肪酸和脂质成分丰富,可以作为促进健康的功能性食品成分的新来源。
The chemical composition of different parts of fish oil from Coregonus peled was analyzed by gas chromatography tandem mass spectrometry,rod thin layer chromatography,nuclear magnetic resonance spectroscopy and liquid chromatography.The results showed that liver is rich in PUFA(account for 30.85%of total fatty acid),especially EPA(account for 10.20%of total fatty acid)and DHA(account for 6.99%of total fatty acid),which are significantly higher than those in fat(PUFA,20.38%;EPA,6.11%;DHA,2.75%)and viscera(PUFA,22.64%;EPA,6.89%;DHA,3.05%).The concentration of tocopherol in liver(546.30μg/g)is markedly higher than that in fat(127.25μg/g)and viscera(157.43μg/g).The concentration of tocotrienol in fat(61.18μg/g)is markedly higher than that in liver(44.44μg/g)and viscera(49.51μg/g).The antioxidant activity of fish oil from Coregonus peled was determined by electron paramagnetic resonance spectroscopy.The results showed that fish oil from liver exhibited good antioxidant activity(EC 50=9.22 mg/mL).The anti-inflammatory activity of fish oil was assessed by enzyme-linked immunoassay.The results showed that the fish oil from Coregonus peled exhibited anti-inflammatory effects by inhibiting the secretion of NO,IL-1β,IL-6 and TNF-α,while promoting the release of IL-10 and IL-13.The results indicated that fish oil from Coregonus peled are rich in functional fatty acid and lipids,which is a potential source of materials for health-promoting food ingredients.
作者
宋国库
秦宁波
夏效东
SONG Guoku;QIN Ningbo;XIA Xiaodong(School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China;National Engineering Research Center of Seafood,Dalian Polytechnic University,Dalian 116034,China)
出处
《大连工业大学学报》
CAS
北大核心
2023年第1期13-20,共8页
Journal of Dalian Polytechnic University
基金
辽宁省科技厅博士启动项目(2019-BS-019).
关键词
高白鲑
鱼油
多不饱和脂肪酸
生育酚
抗氧化活性
抗炎
Coregonus peled
fish oil
polyunsaturated fatty acid
tocopherol
antioxidant activity
anti-inflammation
