摘要
目的 分析珠海市金湾区2018至2019年儿童社区获得性肺炎病例的病原体谱结果,总结病原流行病学特征,为新发病例的诊治提供依据。方法 回顾性分析2018年12月至2019年11月在珠海市金湾区住院,符合儿童社区获得性肺炎诊断的805例患儿咽拭子标本,通过多重PCR和Luminex液相芯片杂交进行13种呼吸道病原体的核酸检测,对结果按照患儿年龄进行分组统计分析,各分组年龄区间为0~6月龄、6~12月龄、1~3周岁、3~7周岁、7周岁以上。结果 580例患儿咽拭子的病原体核酸检测为阳性;235例患儿检出两或以上病原体核酸阳性;检出率最高的前3种病原体分别是肺炎链球菌(SP)、呼吸道合胞病毒(RSV)和肺炎支原体(MP)。RSV在1岁以下低龄患儿中常见;ADV和MP在1岁以上患儿中检出率更高;其他病原体在各年龄组段分布比较平均。大多数季节特异的病原体如SP、HRV、MC、ADV、RSV检出高峰在春季(1~3月份)和冬季(10~12月),但也有少部分如Hi和MP有独立的流行规律,在非检出高峰时段流行。结论 在临床诊断和治疗中,应注意病原体流行的季节性变化、不同年龄组常见病原体的差异以及多种病原体的感染。
Objective To analyze the pathogen spectrum results of community-acquired pneumonia cases among children in Jinwan District,Zhuhai City from 2018 to 2019,and summarize the epidemiological characteristics of the pathogens,to provide a basis for the diagnosis and treatment of new cases. Methods Throat swabs of 805 children hospitalized in Guangdong Provincial People’s Hospital Zhuhai Hospital(Zhuhai Jinwan Central Hospital)from December 2018 to November 2019 were collected,all of whom were diagnosed community acquired pneumonia according to their clinical symptoms. The nucleic acid of 13 respiratory tract pathogens was detected by multiplex PCR and liquid chip technology. The results were analyzed statistically according to the age groups of 0~6 months,6~12 months,1~3 years,3~7 years and >7 years. Results 580throat swabs were detected positive,while 235 were detected two or more pathogens-positive. Among the 13pathogens,the 3 most common pathogenic etiologies were streptococcus pneumoniae(SP)respiratory syncytial virus(RSV)and mycoplasma pneumonia(MP). RSV was popular in children younger than 1 years old while ADV and MP were detected more frequently in children elder than 1 year. And the other 10 pathogens are more evenly distributed across all age groups. Detection peaks of most season-specific pathogen such as SP,HRV,MC,ADV,and RSV are at spring(January-March)and winter(October-December),but Hi and MP have independent epidemiological pattern,which are prevalent during non-detection peak hours. Conclusion In clinical diagnosis and treatment,attention should be paid to the seasonal variation of pathogen prevalence,the difference of common pathogens among age groups and the infection of multiple pathogens.
作者
黄小燕
东建亭
曾宪鍫
HUANG Xiaoyan;DONG Jianting;ZENG Xianqiao(Department of Laboratory Medicine,Department of Guangdong Provincial People's Hospital Zhuhai Hos-pital(Zhuhai Jinwan Central Hospital),Zhuhai,Guangdong,China,519041;Department of Pediatrics,Guangdong Provincial People's Hospital Zhuhai Hospital(Zhuhai Jinwan Central Hospital),Zhuhai,Guang-dong,China,519041;Guangzhou Daan Clinical Laboratory Center,Yunkang Group,Guangzhou,Guang-dong,China,510670)
出处
《分子诊断与治疗杂志》
2023年第1期14-17,共4页
Journal of Molecular Diagnostics and Therapy
