摘要
目的 探讨苦参藜芦配伍合煎毒性化合物变化,为苦参藜芦配伍禁忌的深入研究提供依据。方法 利用超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF/MS)检测苦参藜芦药对配伍禁忌的合煎液和单煎合并液,通过主成分分析法(PCA)、正交偏最小二乘判别法(OPLS-DA)、变量重要性投影(VIP)分析二者特征化合物差异,结合质谱数据库检索对潜在的特征化合物进行快速鉴定。结果 苦参藜芦合煎液中来自藜芦的甾体类生物碱(VSA)含量显著增加,2组比较发现8个化合物存在显著差异,其中合煎液中藜芦新碱(峰3)、藜芦洛明(峰7)、伪芥芬胺(峰2)、藜芦胺(峰6)、伊贝辛(峰4)、3-藜芦酰棋盘花胺(峰9)、茄啶(峰11)含量增加显著,藜芦新碱、藜芦洛明、伪芥芬胺、藜芦胺、伊贝辛、3-藜芦酰棋盘花胺、茄啶可能是二者配伍导致毒副反应的潜在化学标志物。结论 苦参藜芦合煎促进藜芦中VSA溶出,二者配伍禁忌可能与其中多种毒性成分含量增加密切相关,10∶1比例配伍合煎可以初步认为是苦参藜芦反药组合的禁忌条件。
Objective To investigate the changes of toxic compounds in the co-decoction of Sophora flavescens Ait.and Veratrum nigrum Linn.in order to provide data for studies on their incompatibility.Methods UPLC-QTOF/MS was used to detect the co-decoction and single decoction related to the incompatibility Sophora flavescens Ait.and Veratrum nigrum Linn..The differences in characteristic compounds between the two groups were analyzed via principal component analysis(PCA),orthogonal partial least squares discriminant(OPLS-DA) and variable importance projection(VIP).The potential characteristic compounds were quickly identified by means of mass spectrometry database search.Results The contents of veratrum steroid alkaloids(VSA) derived from Veratrum nigrum Linn.increased significantly in the co-decoction of Sophora flavescens Ait.and Veratrum nigrum Linn..A comparison between the two groups showed that there were significant differences in eight chemical components.The contents of veratrosine(peak 3),veralomine(peak 7),pseudojervine(peak 2),veratramine(peak 6),yibeissine(peak 4),3-veratroyl-zygadenine(peak 9),verazine(peak 11) increased significantly,all of which might be potential chemical markers of toxicity and side effects caused by their compatibility.Conclusion The co-decoction of Sophora flavescens Ait.and Veratrum nigrum Linn.can promote the dissolution of VSA.The incompatibility of Sophora flavescens Ait.and Veratrum nigrum Linn.may be closely related to the content increase of several toxic components.The 10∶1 ratio of the co-decoction can be considered to underlie the contraindication of the incompatibility of Sophora flavescens Ait and Veratrum nigrum Linn..
作者
王超
王宇光
马增春
高月
WANG Chao;WANG Yuguang;MA Zengchun;GAO Yue(Anhui Academy of Medical Sciences,Hefei Anhui 230061,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)
出处
《中国药物警戒》
2023年第2期163-167,共5页
Chinese Journal of Pharmacovigilance
基金
国家科技重大专项重大新药创制(2015ZX09501004)
国家自然科学基金资助项目(82192911)
国家中医药传承创新团队项目(ZYYCXTD-C-202009)。
