摘要
小麦雄性不育材料作为一种非常宝贵的种质资源,对小麦杂交育种和杂种优势利用研究具有重要价值。本团队前期从小麦高代品系4167中发现一个雄性不育突变体,将其命名为4167ms,对其进行了不育表型鉴定、遗传分析和分子标记定位研究。结果表明,该突变体表现为花药干瘪不外露,1%I2-KI染色发现突变体花粉粒为不能正常着色的无规则形状,败育彻底。经过多年秋播和春播鉴定,其不育性状稳定,不受光温变化的影响。以4167ms为母本和不同小麦品种杂交得到的正交F1育性完全正常,F2分离群体中可育株与不育株的理论分离比例均符合3∶1;反交(科大342//4167ms/4167)F1结实正常,F2育性分离行中可育株与不育株的分离比例符合3∶1,说明4167ms不育表型受单隐性核不育基因控制,暂命名为ms1t。构建4167ms/中国春F2定位群体,采用BSA法和SSR分子标记连锁分析,获得位于小麦染色体4BS上5个与mslt连锁的SSR标记,其顺序为Xwmc617、Xkd661、Xkd696、ms1t、Xkd495、Xkd393,两侧最近标记Xkd696、Xkd495与ms1t基因的遗传距离分别为3.9和1.9 cM。比对分析中国春物理图谱,ms1t与MS1基因位于相同区域,因此推测4167ms的ms1t可能是MS1的一个隐性等位变异。
As a very valuable germplasm resource,the wheat materials showing male sterile is of significance in the study of wheat hybrid breeding and heterosis utilization.In this study,we identified a male sterility mutant 4167ms from a wheat high generation line 4167,followed by the phenotype classification,genetic analysis and molecular marker mapping.The anthers of the mutant were shriveled and not exposed.The pollens were irregular in shape and failed to be stained with 1%I2-KI,indicating complete sterility of the pollens.Gained from the outcome of field trials over several years,the male sterility were stable without affects due to light and temperature conditions.The F1plants derived from crosses between 4167ms and a number of varieties were completely fertile.The segregation ratio(fertile vs.sterile plants)in F2populations fitted to 3∶1.The F1plants(KD342//4167ms/4167),which were derived from reciprocal cross,were fertile,and the segregation ratio(fertile vs.sterile plants)in derived F2population of segregation was 3∶1.These results indicated that the sterility was controlled by a single recessive nuclear male sterile gene,temporarily designated ms1t.By taking use of a 4167ms/Chinese spring F2mapping population,together with bulked segregant analysis(BSA)and mapping with SSR molecular markers,the ms1t was delimited with five markers(Xwmc617-Xkd661-Xkd696-ms1t-Xkd495-Xkd393)on chromosome 4BS.The genetic distance of the closest flanking markers Xkd696 and Xkd495 to ms1t were 3.9 cM and 1.9 cM,respectively.The comparative analysis based on the physical map of Chinese Spring suggested that ms1t was allocated to the same genetic interval of the former classified ms1 locus,implying ms1t in mutant 4167ms as a novel recessive allele of the MS1gene.
作者
李增辉
王诗音
庞玉辉
马指挥
李家创
王黎明
董普辉
LI Zeng-hui;WANG Shi-yin;PANG Yu-hui;MA Zhi-hui;LI Jia-chuang;WANG Li-ming;DONG Pu-hui(College of Agronomy,Henan University of Science and Technology,Luoyang 471003)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2023年第2期357-364,共8页
Journal of Plant Genetic Resources
基金
国家自然科学基金(U1304320,U1904108)
河南省科技攻关项目(202102110022)。
关键词
小麦
雄性不育
遗传分析
分子标记
wheat
male sterility
genetic analysis
molecular marker
