摘要
MADS-box是植物中一类重要的转录因子,在植物生长发育和信号传导过程中发挥重要作用,特别是在显花植物的花器官分化、开花时间的调节等方面起到重要的调控作用。本研究基于油茶转录组数据,利用生物信息学方法筛选出20个MADS-box转录因子,并对其理化性质、保守结构域、亚细胞定位、二级结构等进行预测分析,同时构建和模式植物拟南芥MADS-box蛋白家族的系统进化树。依据保守结构域将20个CoMADS基因分为TypeⅠ型和TypeⅡ型(MIKC),所编码的蛋白序列长度为89~564 aa,理论等电点在5.75~10.28之间,大部分蛋白为碱性不稳定蛋白,并具有一定的亲水性,氨基酸组成以亮氨酸(Leu)含量最为丰富。所有蛋白均不含有信号肽和导肽,不具有跨膜结构,二级结构以α-螺旋和无规则卷曲为主,均定位于细胞核。聚类分析发现1个TypeⅠ型CoMADS蛋白聚类到拟南芥Mλ亚族,3个TypeⅠ型CoMADS聚类到型Mδ亚族;5个CoMADS蛋白单独聚类,没有归属到拟南芥MADS-box蛋白中;10个MIKC型CoMADS蛋白归属于拟南芥MIKCc型MADS-box蛋白的不同亚家族,其中SVP (CoMADS07, CoMADS08, CoMADS11)和SOC1 (CoMADS13, CoMADS18, CoMADS20)亚族各包含3个成员,有2个成员(CoMADS05, CoMADS10)归属于FLC亚族,AP1/FUL和AG亚族均只有一个成员,分别为CoMADS03和CoMADS05。本研究结果有助于今后进一步探究MADS-box转录因子在油茶生长发育特别是生殖发育调控方面发挥的作用。
The MADS-box family members belong to an important kind of plant transcriptors, which plays an important role in plant growth, development and signal transduction, especially in the regulation of floral organ differentiation and flowering time in flowering plants. This study was based on the transcriptome data of Camellia oleifera, 20 MADS-box transcription factor family members were identified and their protein physicochemical properties, functional domain, subcellular localization and secondary structure were predicted and analyzed. And the phylogenetic tree of MADS-box protein family in C. oleifera and Arabidopsis thaliana were constructed. According to the conserved domains, 20 CoMADS genes were classified into type I and type Ⅱ(MIKC). The amino acid number of the 20 MADS-box proteins ranged from 89 to 564 aa, and the isoelectric point from 5.75 to 10.28, most of CoMADS proteins were basic unstable proteins, and had certain hydrophilicity, Leu was the most abundantly in the consists of amino acids. All of them without a signal peptide, guide peptide and transmembrane structure. Their main secondary structure elements were α-helix and random curl, and they were localized at the nucleus. Cluster analysis showed that 1 MADS-box protein(CoMADS01) in typeⅠ was clustered into mλ subgroup of A.thaliana,and the 3 MADS-box proteins were clustered into mδ subgroup, 5 CoMADS proteins were clustered separately and did not belong to the MADS-box protein subgroup of A. thaliana;10 CoMADS proteins of MIKC type belong to different subfamilies of MIKC type MADS-box proteins in A. thaliana, SVP(CoMADS07, CoMADS08, CoMADS11) and SOC1(CoMADS13, CoMADS18, CoMADS20) subfamily each contain three members, two members(CoMADS05, CoMADS10) belong to FLC subfamily, AP1/FUL and AG subfamily have only one member, CoMADS03 and CoMADS05, respectively. This work will be helpful to further explore the role of MADS-box transcription factors in growth and development in C. oleifera, especially reproductive development.
作者
徐嘉娟
朱亚艳
李芳
杨冰
霍达
许杰
Xu Jiajuan;Zhu Yayan;Li Fang;Yang Bing;Huo Da;Xu Jie(Guizhou Academy of Forestry,Guiyang,550005)
出处
《分子植物育种》
CAS
北大核心
2023年第5期1455-1467,共13页
Molecular Plant Breeding
基金
贵州省科技计划项目(黔科合服企[2018]4003
黔科合人才[2019]5643)
贵州省林业特色产业项目(特林研2020-15)共同资助。
