摘要
为探究添加渔药是否会影响生物絮团氨氮去除能力,在淡水生物絮团反应器中加入常用剂量的氟苯尼考(0.45 mg/L)、多西环素(1.5 mg/L)、磺胺间甲氧嘧啶(4.8 mg/L)、磺胺嘧啶(4.5 mg/L)和恩诺沙星(15 mg/L)5种常用渔药,分析生物絮团去除20 mg/L氨氮过程中的氨氮、亚硝态氮、硝态氮的质量浓度变化,比较各试验组中的菌群结构。试验结果显示:5种渔药在设定剂量条件下对氨氮的去除效果无显著影响,添加氟苯尼考和多西环素的反应器中亚硝态氮质量浓度和持续时间明显高于对照组,磺胺间甲氧嘧啶、磺胺嘧啶和恩诺沙星的反应器中亚硝态氮质量浓度和持续时间明显低于对照组;添加磺胺间甲氧嘧啶、氟苯尼考、多西环素的反应器中多样性指数和丰度与对照组差异不显著(P>0.05),磺胺嘧啶和恩诺沙星的反应器中菌群多样性明显高于对照组(P<0.05),物种丰度明显低于对照组(P<0.05);5种渔药中,15 mg/L恩诺沙星对菌群结构的影响最明显,其次为1.5 mg/L多西环素和0.45 mg/L氟苯尼考,4.8 mg/L磺胺间甲氧嘧啶和4.5 mg/L磺胺嘧啶则影响最小。本研究结果表明,在生物絮凝养殖水体中使用0.45 mg/L氟苯尼考和1.5 mg/L多西环素时需要考虑其可能造成的亚硝态氮积累的影响。
In order to probe for the effect of addition of aquaculture drugs on the ability of bioflocs to remove ammonia nitrogen,conventional doses of florfenicol(0.45 mg/L),doxycycline(1.5 mg/L),sulfamethoxacin(4.8 mg/L),sulfadiazine(4.5 mg/L)and enrofloxacin(15 mg/L)were added into a 10 L plexiglass reactor with a diameter of 20 cm,a height of 50 cm,and a conical bottom containing the biofloc suspension of the tilapia aquaculture system.The dynamics of total ammonia nitrogen(TAN),nitrite nitrogen and nitrate nitrogen in the process of removing 20 mg/L ammonia nitrogen by biological flocs were measured,and the bacterial community structure in each experimental group was compared for 20 days.The reactor containing only the biological floc suspension without the addition of fishery drugs was used as a ontrol group.No significant effect was found in TAN removal for all the five chemicals with their current concentrations under the experimental conditions..There were significantly higher nitrite nitrogen concentration in reactors with florfenicol and doxycycline than that in the control group(P<0.05),with less nitrite nitrogen concentrations in reactors with sulfamethoxacin,sulfadiazine and enrofloxacin than that in the control group,without significant differences in the richness and diversity of bacterial community in the reactors with sulfamethoxacin,florfenicol and doxycycline,and in the control group(P>0.05).Significantly higher diversity of bacterial community was observed in the reactors with sulfadiazine and enrofloxacin than that in the control reactors(P<0.05),with significantly less richness of bacterial community in these two reactors than that in the control reactors(P<0.05).Among the five chemicals,the most significant difference in the bacterial community was found in the reactors with enrofloxacin(15 mg/L),followed by doxycyclinev(1.5 mg/L)and florfenicol(0.45 mg/L),wihtout significant differences in bacterial community on phylum,class and genus levels between the reactors with the other two chemicals compared to the control group(P>0.05).
作者
罗国芝
吴慧芳
郝亚宁
姚妙兰
谭洪新
LUO Guozhi;WU Huifang;HAO Yaning;YAO Miaolan;TAN Hongxin(Research and Development Center of Aquaculture Engineering of Shanghai,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding,Shanghai 201306,China)
出处
《水产科学》
CAS
CSCD
北大核心
2023年第2期206-213,共8页
Fisheries Science
基金
国家自然科学基金资助项目(31202033)。
关键词
生物絮凝
渔药
氨氮
细菌群落
bioflocs
fishery chemical
ammonia nitrogen
bacterial community
