摘要
目的:开发具有抗氧化活性和补钙功能的产品。方法:在固相法合成GYLEQ抗氧化肽的基础上,采用单因素和正交试验优化酪蛋白GYLEQ抗氧化肽与钙离子螯合反应条件,利用红外光谱和扫描电镜表征GYLEQ-Ca螯合物的结构,并分析GYLEQ-Ca螯合物体外抗氧化活性、模拟胃肠液中的持钙率以及对细胞活力的影响。结果:GYLEQ-Ca螯合反应最佳条件为螯合温度50℃,螯合时间40 min, pH 7.5,肽钙质量比4∶1,其中温度对螯合率的影响达到了极显著水平,pH和肽—钙质量比对螯合率的影响达到了显著水平。螯合后产物的红外波谱吸收峰与GYLEQ肽相比发生了改变,扫描电镜观察发现GYLEQ肽为颗粒状,而GYLEQ-Ca螯合物为黏连在一起的网状结构,表明GYLEQ肽与钙离子反应生成了新的化合物。GYLEQ-Ca螯合物的DPPH自由基、羟自由基清除率和还原力高于GYLEQ肽,GYLEQ-Ca螯合物在模拟肠液中持钙率维持在70%以上,且无细胞毒性。结论:GYLEQ-Ca螯合物具有较好的抗氧化活性和钙维持率。
Objective: Preparation of GYLEQ-Ca chelate with antioxidant activity and calcium supplement function. Methods: On the basis of solid-phase synthesis of GYLEQ antioxidant peptide, the conditions of chelation reaction between casein GYLEQ antioxidant peptide and Ca2+were optimized by single factor and orthogonal experiment. The structure of GYLEQ-Ca chelate was characterized by IR and SEM. Meanwhile, the in vitro antioxidant activity, calcium retention in simulated gastrointestinal juice of GYLEQ-Ca chelate and its effect on cell viability were analyzed. Results: The optimum conditions for GYLEQ-Ca chelation reaction were temperature 50 ℃, time 40 min, pH 7.5, and peptide-calcium mass ratio 4∶1. The influence of chelation temperature on the chelation rate reached a very significant level, and the pH and peptide-calcium mass ratio reached a significant level. The infrared absorption peak of the GYLEQ-Ca chelate was changed compared with GYLEQ peptide, and the SEM observation showed that GYLEQ peptide was granular, while GYLEQ-Ca chelate was a network structure adhered together, indicating that GYLEQ peptide reacted with Ca2+to form a new compound. The DPPH, hydroxyl radical scavenging rate and reducing power of GYLEQ-Ca chelate were higher than those of GYLEQ peptide. The calcium retention rate of GYLEQ-Ca chelate in simulated intestinal fluid was maintained at more than 70%, and GYLEQ-Ca chelate has no cytotoxicity. Conclusion: GYLEQ-Ca chelate has good antioxidant activity and calcium maintenance rate.
作者
厉荣玉
厉红
郑鹏
杨成
钱森和
LI Rong-yu;LI Hong;ZHENG Peng;YANG Cheng;QIAN Sen-he(School of Basic Medical Sciences,Wannan Medical College,Wuhu,Anhui 241002,China;Taicang First People s Hospital,Taicang,Jiangsu 215400,China;College of Biological and Food Engineering,Anhui Polytechnic University,Wuhu,Anhui 241000,China)
出处
《食品与机械》
CSCD
北大核心
2023年第2期37-44,共8页
Food and Machinery
基金
皖南医学院校重点项目科研基金(编号:WK2021Z20)
安徽省高校科学研究项目(编号:KJ2021A0840)。
