摘要
为探究鲍鱼内脏酶解物对辣椒素诱导的系统低度炎症模型小鼠炎性因子、肠道菌群及短链脂肪酸的影响,将30只雄性小鼠随机分为6组:空白对照组、辣椒素致炎模型组、鱼油对照组、鲍鱼内脏酶解物低、中、高剂量组(0.30、0.60、1.20 g/(kg·d)),干预7 d后,ELISA法检测小鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10),鲎试剂法测定小鼠血清中细菌脂多糖(LPS)的含量,16S rRNA基因高通量测序和生物信息学方法考察小鼠肠道菌群的结构组成,GC-MS法测定小鼠粪便中短链脂肪酸的含量。结果显示,辣椒素模型组小鼠血清中TNF-α、IL-6和LPS含量显著升高,肠道菌群OTUs数显著降低,菌群各门、属水平比例存在显著变化,其中寡养单胞菌属(Stenotrophomonas)和棒状杆菌属(Corynebactrium)等有害相对丰度显著增加,粪便中的乙酸、丙酸以及总短链脂肪酸含量显著降低,异戊酸含量显著升高,而经鲍鱼内脏酶解物干预后,小鼠血清中IL-1β、IL-6和LPS含量显著降低,肠道中乳杆菌属(Lacbobacillus)、阿洛巴氏菌属(Allobaculum)、拟杆菌属(Bacteroides)和瘤胃球菌属(Ruminococcus)等短链脂肪酸产生相关细菌的相对丰度显著增加,寡养单胞菌属和棒状杆菌属相对丰度显著降低,乙酸、丙酸和总短链脂肪酸的含量显著升高,异戊酸含量显著降低。鲍鱼内脏酶解物可以调节肠道菌群结构和肠道短链脂肪酸组成缓解机体系统低度炎症反应。
Effects of abalone visceral enzymatic hydrolysate on inflammatory factors,intestinal microbial population(IMP)and short chain fatty acids in mice with systemic low-grade inflammation induced by capsaicin were studied.A total of 30 male mice were randomly divided into 6 groups,and blank control group,capsaicin induced inflammatory model group,fish oil control group,low dose,middle dose and high dose groups of abalone visceral hydrolysates(0.30,0.60,1.20 g/(kg·d)).7 days after intervention,the contents of TNF-α,IL-1β,IL-6,IL-10 in mice serum were detected by ELISA,and LPS content in mice serum with TAL.The structure composition of IMP of mice were investigated by 16S rRNA gene high-throughput sequencing and bioinformatics methods,and the content of short-chain fatty acids in mouse feces was determined by GC-MS.After intragastric administration of high dose capsaicin,the contents of TNF-α,IL-6 and LPS in serum of capsaicin model groups increased significantly.The number of OTUs in IMP significantly decreased.The phyla and genera level proportion of IMP changed significantly and the content of acetic acid,propionic acid,as well as short chain fatty acids in feces decreased significantly,however,isovaleric acid increased significantly.Yet after the intervention of abalone visceral enzyme hydrolysate,the contents of IL-1β,IL-6 and LPS in serum decreased significantly,while the abundance of beneficial bacteria such as Lactobacillus,Bacteroides,Allobaculum and Ruminococcus and other bacterial genera of short chain fatty acid related producing bacteria increased significantly.The abundance of harmful bacteria such as Stenotrophomonas and Corynebacterium decreased significantly.The contents of acetic acid,propionic acid and total short chain fatty acids increased significantly and the content of isovaleric acid decreased significantly.The visceral enzymatic hydrolysate of abalone can regulate the structure of IMP and the composition of intestinal shortvchain fatty acids to alleviate the low-grade inflammation reaction of the body system.
作者
何晓彤
王晨
孙力军
孙东方
胡连花
龚丽
房志家
邓旗
HE Xiao-tong;WANG Chen;SUN Li-jun;SUN Dong-fang;HU Lian-hua;GONG Li;FANG Zhi-jia;DENG Qi(Coll.of Food Sci.&Tech.,Guangdong Ocean Uni.,Guangdong Prov.Key Lab.of Aqua.Prod.Process.&Safety,Guangdong Prov.Engin.Lab.for Mar.Bio-Prod.,Guangdong Prov.Engin.Tech.Res.Ctr.of Seafood,Key Lab.of Advanced Process.of Aqua.Prod.of Guangdong Higher Educ.Inst.,Zhanjiang 524088;Guangdong Inst.of Mod.Agric.Equip.,Guangzhou 510630)
出处
《微生物学杂志》
CAS
CSCD
2022年第6期94-102,共9页
Journal of Microbiology
基金
广东省重点领域研发计划项目(2021B0202060001)
广东省现代农业产业技术体系创新团队建设专项资金项目(2020KJ149,2020KJ151)
湛江市科技计划项目(2020A03009)。
关键词
鲍鱼内脏酶解物
辣椒素
系统低度炎症
肠道菌群
短链脂肪酸
abalone visceral enzymatic hydrolysate
capsaicin
systemic low-grade inflammation
intestinal microbial population(IMP)
short chain fatty acids
