摘要
采用高效凝胶过滤色谱法(HPGFC)测定皂角米中多糖组分的含量,以水凝胶色谱柱Ultrahydrogel^(TM) Linear300 mm×7.8 mmid×2为分离柱;以0.1 mol/L硝酸钠为流动相;流速:0.8 mL/min;柱温:40℃。试验表明,多糖组分1#(M_(w)=2 115 637),2#(M_(w)=12 516),3#(M_(w)=418)在质量浓度0.10~5.00 g/L之间,峰面积与质量浓度呈良好的线性关系,相关系数γ为0.999 5,0.999 2,0.999 0(n=5),方法检出限为0.056,0.053,0.050 g/L,加标回收率为96.7%~102.0%,96.0%~101.6%,94.0%~103.6%,相对标准偏差为0.63%~1.81%,0.81%~1.94%,1.01%~2.03%(n=6)。该方法操作简便,具有良好的重复性和回收率,可用于皂角米中多糖组分的检测。
High performance gel filtration chromatography was used to determine the polysaccharide components in sapiens.The hydrogel column Ultra-hydrogel^(TM) Linear 300 mm×7.8 mmid×2 was used as the separation column with the mobile phase of 0.1mol/L sodium nitrate,the flow rate of 0.8 mL/min,and the column temperature at 40℃.Test results show that the polysaccharide components 1#(M_(w)=2 115 637),2#(M_(w)=12 516),3#(M_(w)=418) are in the range of 0.10-5.00 g/L,the peak areas and mass concentrations appear good linear correlation with the correlation coefficient γ of 0.999 5,0.999 2,and 0.999 0(n=5),respectively.The detection limits are 0.056,0.053,and 0.050 g/L,respectively,the recoveries of this method are 96.7%-102.0%,96.0%-101.6%,and 94.0%-103.6%,respectively,and the relative standard deviations are 0.63%-1.81%,0.81%-1.94%,and 1.01%-2.03%(n=6),respectively.The method is simple,has good repeatability and recovery,and can be used for the determination of polysaccharide components in sapiens.
作者
王泽云
张博丽
Zeyun Wang;Boli Zhang(Department of Applied Chemistry,Yuncheng College,Yuncheng,Shanxi 044000,China;Yuncheng Salt Chemical Group Co.,Ltd.,Yuncheng,Shanxi 044000,China)
出处
《日用化学工业（中英文）》
CAS
北大核心
2023年第3期361-364,共4页
China Surfactant Detergent & Cosmetics
基金
运城学院学科建设经费。
关键词
皂角米
高效凝胶过滤色谱法
多糖
Gleditsia sinensis seed
high performance gel filtration chromatography
polysaccharide
