摘要
目的:探讨基于酪蛋白酸钠-葡聚糖糖基化复合物的植物甾醇纳米分散液(PSND)对高脂膳食诱导肥胖小鼠的降血脂和降血糖作用。方法:将SPF级C57BL/6J雄性小鼠随机分成正常对照组(CK组)、高脂饮食组(HFD组)、酪蛋白酸钠-葡聚糖糖基化复合物空载组(N组)、PSND低剂量(L)、中剂量(M)和高剂量(H)干预组,其中L、M、H组小鼠在食用高脂饲料的基础上,同时饮用分别含有0.3,0.5,0.7 mg/mL植物甾醇的纳米分散液,连续饲喂15,16,17周后,分别进行小鼠空腹血糖的测定、葡萄糖耐量实验(GTT)、胰岛素耐量实验(ITT)。18周后结束实验,测定小鼠体质量、皮下脂肪、附睾脂肪和棕色脂肪质量、脂肪系数、肝脏指数、血清生化等指标;测定肝脏胆固醇代谢相关基因3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)、低密度脂蛋白受体(LDL-R)和胆固醇7α-羟化酶(CYP7A1)的mRNA表达水平。结果:经过18周的PSND干预,与高脂饮食(HFD)组相比,低剂量(L,0.3 mg/mL)、中剂量(M,0.5 mg/mL)和高剂量(H,0.7 mg/mL)组小鼠体质量增长量分别降低31.25%,37.38%和49.13%;所有干预组皮下脂肪质量分别降低19.30%,47.37%和60.53%;H组附睾脂肪质量降低26.05%,棕色脂肪质量降低62.5%。PSND的干预抑制白色脂肪细胞的膨大和棕色脂肪白色化;H组肝脏指数、血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)含量均显著下降;H、M组胆固醇合成相关基因HMGCR的表达显著下调,所有干预组胆固醇分解相关基因LDL-R和H组胆固醇分解相关基因CYP7A1的表达显著上调;M、H组小鼠空腹血糖水平显著降低,M、H组葡萄糖耐量和所有干预组胰岛素灵敏度显著提高。结论:基于酪蛋白酸钠-葡聚糖糖基化复合物的PSND能显著降低高脂膳食诱导的肥胖小鼠血脂水平,同时兼具降血糖和保护肝脏的功效。本研究为扩大植物甾醇在食品中的应用和推广提供了实验依据。
Objective:To investigate the effect of phytosterol nano-dispersion(PSND)with sodium caseinate-dextran glycosylation complex on lowing blood lipid and glucose in obese mice induced by high-fat diet.Methods:SPF C57BL/6J male mice were randomly divided into normal control group(CK group),high-fat diet group(HFD group),low(L),middle(M)and high(H)dosing intervention groups.The mice of L,M and H groups separately drank the dispersions containing 0.3,0.5 mg/mL and 0.7 mg/mL of phytosterol nano-dispersion.At the same time,they were fed with high-fat diet.After continuous feeding 15,16 and 17 weeks,the fasting blood glucose,glucose tolerance test(GTT),insulin tolerance test(ITT)were determined,respectively.After 18 weeks,body weight,subcutaneous fat,epididymal fat,brown fat,fat coefficient,liver index,serum biochemistry and other indicators were measured.The mRNA expression of HMG-CoA reductase(HMGCR),low density lipoprotein receptor(LDL-R)and cholesterol 7α-hydroxylase(CYP7A1)in liver were assayed.Results:After 18 weeks of PSND intervention,compared with the HFD group,the gain of weight of mice in the low(L,0.3 mg/mL),middle(M,0.5 mg/mL)and high(H,0.7 mg/mL)groups decreased by 31.25%,37.38%and 49.13%,respectively.The mass of subcutaneous fat decreased by 19.30%,47.37%and 60.53%in three intervention groups,respectively.The mass of epididymal fat and brown fat decreased by 26.05%and 62.5%and in group H.At the same time,white fat cell enlargement and brown fat whiteness were inhibited with the intervention of phytosterol nano-dispersion.In H group,the liver index and contents of total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),glutamic oxalacetic transaminase(AST)and alanine transaminase(ALT)were significantly decreased.The expression of HMGCR(cholesterol synthesis related gene)was down-regulated in M and H groups,while the expression of LDL-R(cholesterol decomposition related gene)in all intervention groups and CYP7A1 in H group in were up-regulated.In M and H groups,the fasting blood glucose level of mice was reduced,and the glucose tolerance was improved as well as the insulin sensitivity in all intervention groups.Conclusion:phytosterol nano-dispersion with sodium caseinate-glucan glycosylation complex has significant effects of decreasing blood lipid and glucose and protecting the liver.This study provides the experimental basis for promoting the application of phytosterol in food.
作者
张晓云
傅栋桁
张一帆
李亚妮
古丽米热·热合满
安妮
梅晓宏
Zhang Xiaoyun;Fu Dongheng;Zhang Yifan;Li Yani;Gulimire Reheman;An Ni;Mei Xiaohong(College of Food Science and Nutrition Engineering,China Agricultural University,Beijing 100083;Key Laboratory of Safety Assessment of Genetically Modified Organism(Food Safety),Ministry of Agriculture and Rural Affairs,Beijing 100083)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2023年第2期131-141,共11页
Journal of Chinese Institute Of Food Science and Technology
基金
国家科技重大专项(2018ZX0801102B-002)。
