摘要
为了解人参灰葡萄孢对氯氟醚菌唑的抗药性情况,采用菌丝生长速率法测定了人参灰葡萄孢对氯氟醚菌唑的敏感性,采用最小抑菌浓度法监测人参灰葡萄孢对氯氟醚菌唑的抗药性。结果表明:氯氟醚菌唑对供试的148株人参灰葡萄孢菌株的EC_(50)值为0.008 4~2.795 8 mg/L,其敏感性频率分布呈连续单峰曲线,经K-S法检验符合正态分布,未出现敏感性明显下降的抗药性菌株群体,EC50平均值为0.174 4 mg/L,可作为人参灰葡萄孢对氯氟醚菌唑的敏感基线;不同地区来源、不同年份分离的人参灰葡萄孢菌株对氯氟醚菌唑的敏感性差异显著(P<0.05);所检测的人参灰葡萄孢菌株对氯氟醚菌唑的抗药性水平多数为敏感菌株和低抗菌株,只有吉林省抚松县和集安市有少量中抗和高抗菌株,抗性频率均在3.70%以下。本研究为氯氟醚菌唑防治人参灰霉病提供了技术依据。
In order to clarify the resistance of ginseng Botrytis cinerea strains to mefentrifluconazole, the sensitivity of B. cinerea to mefentrifluconazole was determined by mycelial growth rate method, and fungicide-resistance of B. cinerea to mefentrifluconazole was monitored with minimum inhibitory concentration method. The range of EC_(50)values of mefentrifluconazolea gainst the tested B. cinerea strains was from 0.008 4 to 2.795 8 mg/L, and the sensitivity frequency distribution showed a continuous unimodal curve, which was in line with the normal distribution by K-S method. There was no resistant population with significantly decreased sensitivity, and its average EC50value of 0.174 4 mg/L can be used as the sensitive baseline of B. cinerea to mefentrifluconazole. There were significant differences in the sensitivity of B. cinerea strains from different geographical regions and different years to mefentrifluconazole. Most of the tested strains of B.cinerea were sensitive or low resistantto mefentrifluconazole, only a small number of medium and high resistant strains were found in Fusong County and Ji’an City of Jilin Province, and the resistance frequency was all less than 3.7%.
作者
马新智
卢宝慧
王若谷
陈长卿
张艳敬
高洁
冯志伟
侯万鹏
孙国刚
李公启
Ma Xinzhi;Lu Baohui;Wang Ruogu;Chen Changqing;Zhang Yanjing;Gao Jie;Feng Zhiwei;Hou Wanpeng;Sun Guogang;Li Gongqi(Jilin Agricultural University,Changchun130118,P.R.China;Jilin Shenwang Plant Protection Science and Technology Co.Ltd.;Insitute of Ginseng Research in Ji’an City)
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2023年第3期136-139,共4页
Journal of Northeast Forestry University
基金
吉林省科技发展计划(20210204047YY)
国家现代农业中药材产业技术体系项目(CARS-21)。
关键词
人参
灰葡萄孢
氯氟醚菌唑
敏感基线
抗药性
Ginseng
Botrytis cinerea
Mefentrifluconazole
Sensitivity baseline
Fungicide-resistance
