摘要
目的回顾分析南京地区体检人群幽门螺杆菌(Helicobacter pylori,Hp)^(13)C呼气试验与免疫分型结果,探讨本地区Hp感染的特征。方法对2022年度在南京市第一医院进行体检人群进行回顾性分析,采用^(13)C呼气试验和免疫印迹法进行Hp抗体分型检测,比较两种方法检测的感染率,以及不同性别、年龄分型结果的情况。结果^(13)C呼气试验与Hp抗体免疫分型阳性率分别为30.52%、54.29%,差异有统计学意义(χ^(2)=635.24,P<0.001)。按性别分析:^(13)C呼气试验男性阳性率为32.95%,女性阳性率为27.62%,差异有统计学意义(χ^(2)=50.456,P<0.001);Hp抗体免疫分型男性阳性率为56.59%,女性阳性率为51.88%,差异有统计学意义(χ^(2)=5.799,P=0.009)。男性感染率高于女性。按年龄分析显示^(13)C呼气试验<45岁组阳性率为29.71%,45~59岁组阳性率为32.17%,≥60岁组阳性率为30.14%,各组差异有统计学意义(χ^(2)=8.731,P=0.013)。Hp分型结果<45岁组阳性率为50.00%,45~59岁组阳性率为57.83%,≥60岁组阳性率为60.05%,组间差异有统计学意义(χ^(2)=22.386,P<0.001),45岁以上年龄组感染率高于45岁以下年龄组。Hp分型结果显示Ⅰ型、Ⅱ型感染率按性别和年龄分组,两者差异无统计学意义(χ^(2)=2.605,P=0.107)、(χ^(2)=3.833,P=0.147)。^(13)C呼气试验与Hp抗体免疫分型联合检测灵敏度为75.24%,特异性为75.39%,总符合率为75.32%。结论南京地区体检人群幽门螺杆菌男性感染率高于女性,45岁以上人群感染率偏高,免疫分型结果在不同分组间差别无统计学意义。
Objective This study retrospectively analyzed the results of^(13)C breath test and immune typing of Helicobacter pylori(Hp)of physical examination population in Nanjing area.Methods Subjects who underwent physical examination in Nanjing First Hospital in 2022 were selected for the study.The^(13)C breath test and western blot were conducted to detect Hp infection of enrolled participants,and we compared the infection rate of the two methods,as well as the results among different subgroups of gender and age cohorts.Results The positive rates of^(13)C breath test and Hp antibody immune typing were 30.52%and 54.29%,respectively with a significant difference(χ=635.24,P<0.001).Subgroup analysis by gender revealed that the positive rate of^(13)C breath test was 32.9%in males and 27.6%in females(χ=50.456,P<0.001),and the positive rate of Hp antibody was 56.6%in male and 51.9%in female with a significant difference(χ=5.799,P=0.009),which indicated a higher infection rate among men than women.Furthermore,subgroup analysis by age revealed that the positive rate of^(13)C breath test was 29.7%in<45 years old group,32.2%in 45-59 years old group and 30.1%in≥60 years old group with a significant difference(χ=8.731,P=0.013).The positive rate of Hp antibody immune typing was 50.0%in the<45 years old group,57.8%in the 45-59 years old group,and 54.3%in the≥60 years old group,showing a significant difference among groups(χ^(2)=22.386,P<0.001),and the infection rate of the group over age 45 was higher than that of the group under age 45.There was no significant difference between Hp-I and Hp-II infection rates by sex(χ=2.605,P=0.107)and age(χ=3.833,P=0.147).The sensitivity and specificity of^(13)C breath test combined with Hp antibody immunotyping were 75.24%and 75.39%,respectively,and the total coincidence rate was 75.32%.Conclusion The infection rate of Hp in males is higher than that in females,and the infection rate is higher in population over 45 years old.There is no difference in the results of immune typing among different groups.
作者
王刚
张振玉
梅燕萍
王一杉
WANG Gang;ZHANG Zhenyu;MEI Yanping;WANG Yishan(Department of Medical Laboratory,Nanjing Medical University,Nanjing 210006,China;Department of Digestive,Nanjing First Hospital,Nanjing Medical University,Nanjing 210006,China)
出处
《标记免疫分析与临床》
CAS
2023年第1期57-60,169,共5页
Labeled Immunoassays and Clinical Medicine
基金
南京市卫生科技发展专项资金项目(编号:YKK22116)。
