摘要
土壤微生物在森林生态系统能量流动和物质循环过程中发挥着不可替代的作用。兴安落叶松(Larix gmelinii)是我国大兴安岭地区的优势树种,而阿尔山地区是其在我国分布的最南端,受气候变暖影响较为显著,因此探索兴安落叶松林下土壤微生物群落结构对了解和维护我国东北地区森林生态系统的稳定有重要意义。在本研究中,我们从阿尔山白狼镇、天池镇采集兴安落叶松林下土样,通过高通量测序技术,依据97%相似性原则,将细菌序列划分为5,163个可操作分类单位(operational taxonomic units,OTUs),真菌序列划分为2,439个OTUs,其中门水平优势细菌为放线菌门、变形菌门、疣微菌门、绿弯菌门和酸杆菌门,优势真菌为担子菌门和子囊菌门;属水平上,优势细菌为Candidatus_Udaeobacter,优势真菌属为丝盖伞属(Inocybe)、蜡壳耳属(Sebacina)、Piloderma和棉革菌属(Tomentella)。天池镇的细菌多样性显著高于白狼镇,而真菌多样性在两地间无显著差异。土壤pH和阳离子交换量是驱动土壤细菌多样性的主要因子,而土壤全氮和有机碳是驱动真菌多样性的主要因子。土壤全氮、有机碳、速效磷和阳离子交换量是影响土壤细菌群落组成的重要理化因子,而土壤理化性质对真菌群落组成的影响不显著。绿弯菌门的相对多度与pH显著正相关;担子菌门与土壤全氮和有机碳显著负相关,而子囊菌门与土壤全氮、有机碳和阳离子交换量显著正相关。
Aims:Soil microorganisms play an irreplaceable role in the energy flow and material cycle of forest ecosystems.Larix gmelinii is the dominant tree species in the Greater Khingan Mountains of China,and the Aershan area is the southernmost point of its distribution in China,which is significantly affected by climate warming.Exploring the soil microbial community structure under L.gmelinii forest is of great significance to understand and maintain the stability of forest ecosystem in Northeast China.Method:In this study,we collected soil samples from Bailang Town and Tianchi Town in the Aershan area and used high-throughput sequencing technique to analyze the soil bacterial and fungal communities in two forest stands.Results:The results showed that(1)5,163 bacterial operational taxonomic units(OTUs)and 2,439 fungal OTUs were obtained according to a 97%sequence similarity level.The diversity of bacteria was higher in the Tianchi area than in the Bailang area,but no significant difference in fungal diversity between the two sites.(2)The dominant phyla of bacteria were Actinobacteriota,Proteobacteria,Verrucomicrobiota,Chloroflexi,and Acidobacteriota,and the dominant phyla of fungi were Basidiomycota and Ascomycota.The dominant bacterial genus was Candidatus_Udaeobacter,and the dominant genera of fungi were Inocybe,Sebacina,Piloderma and Tomentella.(3)The correlation analysis indicated that soil p H and cation exchange capacity were the main factors driving soil bacterial species diversity,and soil total nitrogen and organic carbon were the main factors driving fungal species diversity.(4)Soil total nitrogen,organic carbon,available phosphorus and cation exchange capacity were important factors affecting soil bacterial community composition,while fungal community composition was not significantly affected by the physical and chemical properties of the soil.(5)The relative abundance of Chloroflexi was significantly correlated with p H;the relative abundance of Basidiomycota was significantly correlated with soil total nitrogen and organic carbon,while that of Ascomycota was significantly correlated with soil total nitrogen,organic carbon and cation exchange capacity.Conclusion:The results of this study indicate that soil physicochemical properties have a significant impact on the soil microbial community structure in L.gmelinii forest within the Aershan area,and the diversity of bacterial and fungal communities also plays an integral role in maintaining the soil ecology and functional environment in this area.
作者
赵雯
王丹丹
热依拉·木民
黄开钏
刘顺
崔宝凯
Wen Zhao;Dandan Wang;Mumin Reyila;Kaichuan Huang;Shun Liu;Baokai Cui(School of Ecology and Nature Conservation,Beijing Forestry University,Beijing 100083)
出处
《生物多样性》
CAS
CSCD
北大核心
2023年第2期151-162,共12页
Biodiversity Science
基金
国家自然科学基金(31870008)
北京林业大学杰出青年人才培育项目(2019JQ03016)。
关键词
兴安落叶松
土壤微生物
高通量测序
群落结构
多样性
Larix gmelinii
soil microorganism
high-throughput sequencing
community structure
diversity
