摘要
为调查青海省民和县玉米叶斑病病原菌种类,从该地区玉米种植地采集具有典型叶斑病症状的玉米病叶,通过叶斑病症状观察分类,采用组织分离法获得菌株,并从分离的菌株中选取具有代表性的菌株进行分子鉴定.结果表明,青海省民和县玉米种植地玉米叶斑病症状主要分为3类,第1类病斑不规则,较大,呈现褐黄色;第2类病斑呈长条形,中央部分呈现黄褐色,边缘是紫褐色或深褐色,有2~3层同心轮纹,且能侵染叶脉部分;第3类病斑呈长方形或菱形,病斑较大.共分离到14株菌株,经rDNA-ITS序列分析发现,引起玉米叶斑病的病原菌分别为交链格孢(Alternaria alternat)、A.burnsii、细级链格孢(A.tenuissima)、层出镰刀菌(Fusarium proliferatum)、三线镰刀菌(F.tricinctum)和F.longifundum.引起青海省民和县玉米叶斑病的病原菌分别隶属于2属6种,病原菌主要为链格孢属真菌.
To investigate the pathogen species of silage maize leaf spot in Minhe County, the diseased leaves with typical symptoms were collected from the corn planting area. The diseased leaves were classified according to the symptoms. The strains of pathogen were obtained by tissue isolation method, and the isolates were identified by molecular biology method. The results showed that there were three types of leaf spots observed in Minhe County. The first type of spot was irregular, large lesions, in brownish yellow color. The second type of spot was long strip, yellow brown in the center, or purple brown or dark brown in the edge, sometimes with 2~3 concentric rings, and the vein could be infected. The third kind of lesions were oval, slightly larger, yellow, scattered on the leaves. Fourteen isolates were isolated and identified by rDNA-ITS sequence analysis as Alternaria alternat, A. burnsii, A. tenuissima, Fusarium proliferatum, F. tricinctum and F. longifundum. The pathogens caused corn leaf spot in Minhe County belonged to 2 genera, 6 species, respectively. The main pathogens are Alternaria.
作者
王杰
王海春
祁鹤兴
刘欣悦
常建萍
杜雪花
芦光新
WANG Jie;WANG Haichun;QI Hexing;LIU Xinyue;CHANG Jianping;DU Xuehua;LU Guangxin(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Head Station of Grassland of Qinghai Province,Xining 810003,China)
出处
《植物医学》
2023年第1期44-49,共6页
Plant Health and Medicine
基金
2022年中央财政林业和草原生态保护恢复资金项目“青海省牧草病害种类及分布调查”
2021年度青海省/昆仑英才“高端创新创业人才”计划项目。
