摘要
目的探讨基于GEO数据库分析黄褐斑基因表达的差异性,并对差异表达基因调控的功能通路进行分析。方法将2021年4月作为时间截点,基于GEO数据库筛选黄褐斑相关基因表达谱,选择“chloasma”为筛选条件,“human”为物种类型。使用GEO2R对黄褐斑差异基因表达情况进行分析,并对通路富集情况做KEGG分析。使用蛋白间相互作用(PPI)找出关键基因及蛋白靶标,分析差异基因表达情况。结果根据GEO数据库共筛选出差异基因34个,其中上调基因16个、下调基因18个。KEGG显示黄褐斑有13条显著差异性富集信号通路(P<0.05),主要与黑色素生成、多种信号通路、Gap连接等有关。GO功能分析显示前30个显著富集条目中,生物过程包括酶联受体蛋白信号通路、膜受体酪氨酸激酶信号通路等,细胞组分包括核内腔、核仁、细胞器内腔等,分子功能包括结合酶、依赖DNA的转录正调节等。STRING分析显示,在PPI网络中提取包含2453种基因的核心网络中排名前5的基因为TYRP1、GDF15、MITF、NRF2和Beclin1,以上5个基因是黄褐斑患者表达的最关键基因,与黄褐斑的发病有关。结论TYRP1、GDF15、MITF、NRF2、Beclin1是影响黄褐斑形成的主要基因,可能通过多种信号通路、受体结合、DNA转录调节等途径影响黑色素的代谢与沉积,导致了黄褐斑的发生发展。
Objective To explore the differences of gene expression in chloasma based on GEO database and to analyze the functional pathways regulated by differentially expressed genes.Methods April 2021 was taken as the time cut-off point,chloasma related gene expression profiles were screened based on GEO database,"chloasma"was selected as the screening condition,"human"as species type.GEO2R was used to analyze the differential gene expression of chloasma,and KEGG analysis was performed for pathway enrichment.Key genes and protein targets were identified by protein-protein interaction(PPI),and differential gene expression was analyzed.Results According to the GEO database,a total of 34 differential genes were screened,including 16 up-regulated genes and 18 down-regulated genes.KEGG showed that there were 13 significantly enriched signaling pathways in chloasma(P<0.05),mainly related to melanin generation,a variety of signal pathways,Gap connection and so on.GO functional analysis showed that among the top 30 significantly enriched entries,biological processes included enzyme-linked receptor protein signaling pathway,membrane receptor tyrosine kinase signaling pathway,cell components included nuclear lumen,nucleolus,organelle lumen,and molecular functions included binding enzymes and DNA-dependent transcriptional upregulation,and the like.STRING analysis showed that the top 5 genes in the core network containing 2453 genes extracted from the PPI network were TYRP1,GDF15,MITF,NRF2 and Beclin1,the above five genes were the most critical genes expressed in patients with chloasma and were related to the pathogenesis of chloasma.Conclusion TYRP1,GDF15,MITF,NRF2,and Beclin1 are the main genes that affect the formation of chloasma,which may affect the metabolism and deposition of melanin through various signaling pathways,receptor binding,DNA transcription regulation and other pathways,leading to the occurrence and development of chloasma.
作者
张迁
刘娟
丁洪青
徐蕴杰
张博
陈丽梅
赵娜
ZHANG Qian;LIU Juan;DING Hongqing;XU Yunjie;ZANG Bo;CHEN Limei;ZHAO Na(Chinese Pharmacy,Hebei Cangzhou Integrated Traditional Chinese and Western Medicine Hospital,Cangzhou,Hebei 061000,China;Chinese Pharmacy,Hebei Langfang Hospital of Traditional Chinese Medicine,Langfang,Hebei 065000,China;Department of Traditional Chinese Medicine,Hebei Cangzhou Integrated Traditional Chinese and Western Medicine Hospital,Cangzhou,Hebei 061000,China;Department of Dermatology,Hebei Cangzhou Integrated Traditional Chinese and Western Medicine Hospital,Cangzhou,Hebei 061000,China)
出处
《国际检验医学杂志》
CAS
2023年第6期704-707,共4页
International Journal of Laboratory Medicine
基金
河北省中医药管理局科研计划项目(2021332)。
