摘要
目的:探讨芍药苷(PF)调控缺氧诱导因子-1α(HIF-1α)/血管内皮生长因子(VEGF)/血管内皮生长因子受体2(VEGFR2)对难愈性溃疡大鼠创面愈合的影响和作用机制。方法:将60只SD大鼠随机分为假手术(Sham)组、模型(Model)组、PF组(30mg/kg,2次/d)、PX-478组(100mg/kg HIF-1α/VEGF/VEGFR2通路抑制剂PX-478,1次/d)、PF+PX-478组,每组12只,持续2周。通过一般创面注射肌注氢化可的松制成大鼠难愈性创面模型,用相机结合Image J软件计算各组大鼠创面的愈合率;用ELISA法检测大鼠血清中氧化应激指标;HE染色检测大鼠创面病理变化;TUNEL染色检测创面组织细胞凋亡;RT-qPCR检测大鼠创面组织转化生长因子β1(TGF-β1)、血小板衍生因子(PDGF)表达情况;Western blotting法检测大鼠创面组织HIF-1α/VEGF/VEGFR2通路相关蛋白表达。结果:与Sham组相比,Model组大鼠创面成纤维细胞和毛细血管稀疏,愈合率、SOD、GSH-PX水平、TGF-β1、PDGF mRNA水平及HIF-1α、VEGF、VEGFR2蛋白水平显著降低(P<0.05),MDA水平、创面组织细胞凋亡率显著升高(P<0.05);与Model组比较,PF组大鼠创面成纤维细胞和毛细血管较密(P<0.05),创面愈合率、SOD、GSH-PX水平、TGF-β1、PDGF mRNA水平及HIF-1α、VEGF、VEGFR2蛋白水平显著增加(P<0.05),MDA水平、创面组织细胞凋亡率显著降低(P<0.05),PX-478组对应指标呈现相反趋势(P<0.05);与PF组相比,PF+PX-478组大鼠创面成纤维细胞和毛细血管稀疏(P<0.05),愈合率、SOD、GSH-PX水平、TGF-β1、PDGF mRNA水平及HIF-1α、VEGF、VEGFR2蛋白水平显著降低(P<0.05),MDA水平、创面组织细胞凋亡率显著升高(P<0.05)。结论:PF可能通过激活HIF-1α/VEGF/VEGFR2通路改善难愈性溃疡大鼠创面愈合。
Objective:To investigate the influence and mechanism of paeoniflorin(PF)on wound healing in rats with refractory ulcer by regulating hypoxia-inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)/vascular endothelial growth factor receptor 2(VEGFR2).Methods:Sixty SD rats were randomly grouped into Sham operation(Sham)group,Model group,PF group(30 mg/kg,twice a day),PX-478 group(100 mg/kg HIF-1α/VEGF/VEGFR2 pathway inhibitor PX-478,once a day),and PF+PX-478 group,12 animals in each group,twice a day,for 2 weeks.The rat model of refractory wound was made by injecting hydrocortisone into the general wound surface.The camera combined with Image J software was used to calculate the wound healing rate of each group of rats;ELISA method was used to detect oxidative stress indicators in rat serum;HE staining was used to detect the pathological changes of rat wounds;TUNEL staining was used to detect apoptosis in wound tissue;RT-qPCR was used to detect the expressions of transforming growth factorβ1(TGF-β1)and platelet-derived factor(PDGF)in rat wound tissue;Western blotting was used to detect the expression of HIF-1α/VEGF/VEGFR2 pathway-related proteins in rat wound tissue.Results:Compared with the Sham group,the wound fibroblasts and capillaries of the rats in the Model group were sparse,the healing rate,levels of SOD,GSH-PX,mRNA levels of TGF-β1,PDGF,and protein levels of HIF-1α,VEGF,VEGFR2 were obviously decreased(P<0.05),the level of MDA and the apoptosis rate of wound tissue were obviously increased(P<0.05);compared with the Model group,the wound fibroblasts and capillaries of the rats in the PF group were denser(P<0.05),the healing rate,levels of SOD,GSH-PX,mRNA levels of TGF-β1,PDGF,and protein levels of HIF-1α,VEGF,VEGFR2 were obviously increased(P<0.05),the level of MDA and the apoptosis rate of wound tissue were obviously decreased(P<0.05),the corresponding indicators in the PX-478 group showed opposite trends(P<0.05);compared with the PF group,the wound fibroblasts and capillaries of the rats in the PF+PX-478 group were sparse(P<0.05),the healing rate,levels of SOD,GSH-PX,mRNA levels of TGF-β1,PDGF,and protein levels of HIF-1α,VEGF,VEGFR2 were obviously decreased(P<0.05),and the level of MDA and the apoptosis rate of wound tissue were obviously increased(P<0.05).Conclusion:PF may improve wound healing in rats with refractory ulcer by activating the HIF-1α/VEGF/VEGFR2 pathway.
作者
吴熙
陈高
WU Xi;CHEN Gao(Xiaogan Hospital Affiliated to Wuhan University of Science and Technology/Xiaogan Central Hospital,Hubei Xiaogan 432000,China)
出处
《河北医学》
CAS
2023年第3期369-374,共6页
Hebei Medicine
基金
2022年度孝感市自然科学计划项目,(编号:XGKJ2022010010)。
关键词
芍药苷
难愈性溃疡
创面愈合
缺氧诱导因子-1α
血管内皮生长因子
血管内皮生长因子受体2
Paeoniflorin
Refractory ulcer
Wound healing
Hypoxia-inducible factor-1α
Vascular endothelial growth factor
Vascular endothelial growth factor receptor 2
