摘要
黄酮类成分具有较好的降血压降血脂作用,为了解桑枝中芦丁、桑色素、山柰酚、异槲皮苷、桑辛素等黄酮类成分的含量情况,采用超高效液相色谱法(ultra performance liquid chromatography,UPLC)对重庆9个产地、3个种类(桑、鸡桑、华桑)的18批桑枝进行黄酮成分的测定。实验结果显示,芦丁、桑色素、山柰酚、异槲皮苷、桑辛素在各自质量浓度范围内,峰面积和浓度呈现出良好的线性关系,相关系数R为0.9995~0.9998。18批桑枝中均检测出5种黄酮类物质,但其在含量上存在较大差异,五种黄酮类成分的平均含量由高到低为桑辛素>桑色素>芦丁>异槲皮苷>山柰酚,含量最高的桑辛素平均含量为135.154μg/g,含量最低的山柰酚平均含量为6.929μg/g。不同种类桑枝中5种黄酮类物质含量差异较大,华桑中黄酮总含量最高。本研究能为深入研究桑枝的药用价值提供理论依据以及数据资料参考,为临床上降血压降血脂的治疗提供药物来源参考。
Flavonoids have a good effect of lowering blood pressure and blood lipids.In order to explore the contents of rutin,morin,kaempferol,isoquercitrin,and morusin in mulberry branches,ultra performance liquid chromatography(UPLC)was used to determine the flavonoids in 18 batches of mulberry branches of three spieces(Morus alba L.,M.australis Poir.,M.cathayana Hemsl.)from nine places of origin in Chongqing.The result showed that there was a good linear relationship between peak area and concentration of rutin,morin,kaempferol,isoquercitrin and morusin within their respective mass concentration range,and the correlation coefficient R was 0.9995-0.9998.The five flavonoids were detected in all 18 batches of samples,while there were great differences in their contents.The average content of the five flavonoids from high to low was morusin>morin>rutin>isoquercitrin>kaempferol.The highest was the average content of morusin,which was 135.154μg/g,and the lowest was the average content of kaempferol which was 6.929μg/g.There were great differences in the contents of flavonoids between spieces,and the total flavonoids content in the M.cathayana was the highest.This study can provide theoretical basis and data reference for the in-depth research of the medicinal value of mulberry branches,and provide drug source reference for the clinical treatment of reducing blood pressure and blood lipids.
作者
张椿翊
邓乔晟
郭子晗
乔中华
黄小兰
王森
周游
周浓
ZHANG Chun-yi;DENG Qiao-sheng;GUO Zi-han;QIAO Zhong-hua;HUANG Xiao-lan;WANG Sen;ZHOU You;ZHOU Nong(College of Biology and Food Engineering,Chongqing Three Gorges University;Chongqing Wanzhou FDA Inspection Institute,Chongqing 404100,China;School of Pharmacy,Zunyi Medical University,Zunyi 563000,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第3期372-378,共7页
Natural Product Research and Development
基金
重庆市教委项目(KJQN202101231)。
关键词
桑枝
黄酮
超高效液相色谱
含量测定
mulberry branches
flavonoids
UPLC
content determination
