摘要
从外植体选择、外植体表面灭菌、组培途径、生根移栽等4个环节综述铁线莲组织培养研究结果,归纳了外植体类型、基因型、植物生长调节剂种类和配比、培养条件等因素对铁线莲再生的影响。结果表明,基因差异和生长调节剂调控对铁线莲组织培养的影响最为显著;并针对铁线莲组织培养研究中存在的问题提出展望。研究结果可为铁线莲再生体系构建和工厂化育苗提供参考。
The article reviews the results of tissue culture research on Clematis from four aspects,including explant selection,surface sterilization of explants,culturing route and rooting.And the effects of explant type,genotype,plant growth regulators′type and ratios and culture conditions were summarized.The results show that genetic differences and growth regulator had the most significant effects on Clematis tissue culture.Finally,the article presented perspectives on the problems in Clematis′tissue culture.The results can be used as a reference for the construction of Clematis′regeneration system and industrial seedling culture.
作者
王超
丁世民
窦京海
李常英
张二海
WANG Chao;DING Shimin;DOU Jinghai;LI Changying;ZHANG Erhai(Weifang Vocational Academy,WeiFang 262737,Shandong,China)
出处
《福建林业科技》
2023年第1期142-148,共7页
Journal of Fujian Forestry Science and Technology
关键词
铁线莲
组织培养
研究进展
Clematis
tissue culture
research progress
