摘要
香菇单核体菌株在传统PDA培养基上生长时具有生长缓慢、容易老化等问题,本研究以1株香菇双核体Y0040以及相对应的2株单核体(Y0040-1和Y0040-3)为研究材料,通过添加不同比例木屑粉的PDA培养基筛选适合香菇单核体生长的配比,结果表明添加木屑能够显著促进单核体菌丝的生长,最适添加比例为2%。将Y0040-1和Y0040-3在PDA和2%木屑PDA上培养后进行转录组表达谱差异分析,结果显示Y0040-1和Y0040-3两个单核菌株在木屑-PDA培养基上生长有1066个共有的差异表达基因,进一步对其注释发现,这些差异基因在细胞结构合成以及碳水化合物代谢等途径上得到富集。同时1066个共有的差异基因中有113个共上调,富集于氧化还原反应,267个共下调主要富集于蛋白质折叠和去折叠等途径。进一步对1066个差异基因进行CAZYmes家族和木质纤维素酶分析,发现有36个家族基因差异表达,包括了4个多铜氧化酶、6个β-葡萄糖苷酶和2个内β-1,4-葡聚糖酶,其中多铜氧化酶基因表达在木屑培养基上都显著上升。木质纤维素降解酶基于氧化还原反应等将木质素降解为菌丝体生长发育所必需的小分子单糖,可能是木屑PDA培养基促进菌丝生长的原因之一。
The monokaryotic strains isolated from dikaryotic Lentinula edodes cultured on PDA medium usually grow slowly and age easily. In this study, one dikaryotic strain Y0040 and two derivative monokaryotic strains, Y0040-1 and Y0040-3, cultured on PDA with addition of sawdust showed significant promotion of the growth, especially on the medium with 2% sawdust.Monokaryotic strains Y0040-1 and Y0040-3 with the highest growth rate were used for transcriptome analysis culturing on PDA and 2% sawdust-PDA. The results showed that the expression profiles were different between the strains growing on PDA and sawdust-PDA.Compared with the strains growing on PDA, Y0040-1 and Y0040-3 strains growing on sawdust-PDA shared a total of 1 066 different expression genes(DEGs). The GO annotations of the 1 066 DEGs revealed that they were enriched in cell structure synthesis and carbohydrate metabolisms. Among the 1 066 DEGs, 113 co-up regulated genes were enriched in oxidoreductase activity, and 267 co-down regulated genes were enriched in protein folding and unfolding. Further analysis of CAZYmes family and lignocellulose degrading genes revealed that a total of 36CAZYmes family genes expressed differently, including four multicopper oxidase genes, two endo-beta-1,4-glucanase genes and six β-glucosidase genes. The expression of multicopper oxidase genes was higher in the strains growing on sawdust-PDA than that in the strains growing on PDA. The high expression of lignocellulose degrading enzyme genes in the two strains might be beneficial to degrade lignocellulose for nutrient absorption for promoting mycelium growth of monokaryotic strains.
作者
侯娣
周陈力
李燕
杨瑞恒
鲍大鹏
HOU Di;ZHOU Chenli;LI Yan;YANG Ruiheng;BAO Dapeng(College of Food Sciences,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Edible Fungal Resources and Utilization(South),National Engineering Research Center of Edible Fungi,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2023年第2期507-519,共13页
Mycosystema
基金
上海市“科技创新行动计划”项目(19391901400)
国家自然科学基金(31800015)。
关键词
香菇
单核体
培养基优化
CAZYmes家族
木质纤维素酶
Lentinula edodes
monokaryotic strains
medium optimization
CAZYmes family
lignocellulose enzymes
