摘要
综合运用硅胶柱色谱、ODS柱色谱、Sephadex LH-20柱色谱、制备薄层色谱、反相HPLC等多种现代色谱学分离手段,从铁皮石斛Dendrobium officinale茎水提后的95%乙醇提取物中分离得到11个化合物。根据波谱学方法(MS、1D-NMR、2D-NMR)结合旋光数据、计算ECD鉴定了上述化合物的结构,分别为dendrocandin Y(1)、4,4′-二羟基联苄(2)、3-羟基-4′,5-二甲氧基联苄(3)、3,3′-二羟基-5-甲氧基联苄(4)、3-羟基-3′,4′,5-三甲氧基联苄(5)、crepidatin(6)、格链孢醇(7)、4-羟基-3-甲氧基苯丙酮(8)、3-羟基-4,5-二甲氧基苯丙酮(9)、auriculatum A(10)和hyperalcohol(11)。其中化合物1为新的联苄衍生物,化合物2、7~11为首次从石斛属植物中分离得到,化合物6为首次从铁皮石斛中分离得到。化合物3~6显示出较强的ABTS自由基清除能力,IC50为3.11~9.05μmol·L^(-1),表明具有抗氧化活性。化合物4对α-葡萄糖苷酶显示出明显的抑制作用,IC50为17.42μmol·L^(-1),表明具有降糖活性。
Eleven compounds were isolated from the 95% ethanol extract of the stems of Dendrobium officinale after water extraction by various modern chromatographic techniques, such as silica gel column chromatography(CC), octadecyl-silica(ODS) CC, Sephadex LH-20 CC, preparative thin layer chromatography(PTLC) and preparative high performance liquid chromatography(PHPLC). According to spectroscopic analyses(MS, 1D-NMR, 2D-NMR) combined with optical rotation data and calculated electronic circular dichroism(ECD), their structures were identified as dendrocandin Y(1), 4,4′-dihydroxybibenzyl(2), 3-hydroxy-4′,5-dimethoxybibenzyl(3), 3,3′-dihydroxy-5-methoxybibenzyl(4), 3-hydroxy-3′,4′,5-trimethoxybibenzyl(5), crepidatin(6), alternariol(7), 4-hydroxy-3-methoxypropiophenone(8), 3-hydroxy-4,5-dimethoxypropiophenone(9), auriculatum A(10) and hyperalcohol(11). Among them, compound 1 was a new bibenzyl derivative;compounds 2 and 7-11 have not been previously reported from Dendrobium plants;compound 6 was reported from D.officinale for the first time. Compounds 3-6 exhibited potent antioxidant activity with IC50values of 3.11-9.05 μmol·L^(-1) in ABTS radical scavenging assay. Compound 4 showed significant inhibitory effect on α-glucosidase, with IC50value of 17.42 μmol·L^(-1), indicating that it boasted hypoglycemic activity.
作者
孟威同
孟晓
牛丽婷
章思思
欧阳春杰
丁春花
朱玲娟
张雪
MENG Wei-tong;MENG Xiao;NIU Li-ting;ZHANG Si-si;OUYANG Chun-jie;DING Chun-hua;ZHU Ling-juan;ZHANG Xue(School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;Xiamen Eagledon Pharmaceutical Co.,Ltd.,Xiamen 361000,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2023年第3期700-706,共7页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2017YFC1702204)。
关键词
铁皮石斛
化学成分
结构鉴定
抗氧化活性
降糖活性
Dendrobium officinale
chemical constituents
structure elucidation
antioxidant activity
hypoglycemic activity
