摘要
目的了解致病性弧菌在沧州市水产品中的污染状况,为食源性疾病防控和食物中毒监测提供数据支持。方法采用随机抽样的方法采集2021年沧州市水产品样品,按照《国家食品污染和有害因素风险监测工作手册》中监测方法,对副溶血性弧菌等4种致病性弧菌进行检测,对霍乱弧菌和副溶血性弧菌进行基因检测,确定其毒力基因,并对霍乱弧菌进行血清分型。结果220份水产品中74份检出致病性弧菌,检出率为33.64%。其中副溶血性弧菌检出率为22.73%,溶藻弧菌检出率为7.27%,霍乱弧菌检出率为2.73%,创伤弧菌检出率为0.91%。分离出的50株副溶血性弧菌均携带tlh基因型,未携带tdh和trh基因;6株霍乱弧菌都为非O1/O139群霍乱弧菌,ctx毒力基因为阴性。结论2021年沧州市部分水产品中存在致病性弧菌污染,以副溶血性弧菌和溶藻弧菌为主。市场监管部门应加强对各类水产品的监管力度,降低致病性弧菌引发的食源性疾病风险。
Objective To investigate the pollution status of pathogenic Vibrio in aquatic products in Cangzhou City,provide data support for the prevention and control of foodborne diseases and monitoring of food poisoning.Methods The samples of aquatic products in Cangzhou City in 2021 were collected by random sampling.According to the methods of National work manual for risk monitoring of food contamination and harmful factors,four kinds of pathogenic Vibrio were detected,the virulence genes of Vibrio cholerae and Vibrio parahaemolyticus were determined,and the Vibrio cholerae was serotyped.Results The pathogenic Vibrio was detected in 74 of 220 aquatic products,and the detection rate was 33.64%.The detection rate of Vibrio parahaemolyticus,Vibrio alginolyticus,Vibrio cholerae and Vibrio vulnificus was 22.73%,7.27%,2.73%and 0.91%,respectively.All 50 strains of Vibrio parahaemolyticus isolated carried the tlh genotype,but did not carry the tdh and trh genes.The six strains of Vibrio cholerae were non-O1/O139 group,and the ctx virulence gene was negative.Conclusions In 2021,there is pathogenic Vibrio contamination in some aquatic products in Cangzhou City,mainly Vibrio parahaemolyticus and Vibrio alginolyticus.The market supervision department should strengthen the supervision of various aquatic products to reduce the risk of foodborne diseases caused by pathogenic Vibrio.
作者
吴秉伦
张瑞
毕红杰
卢伟
胡倩倩
王巨忠
WU Bing-lun;ZHANG Rui;BI Hong-jie;LU Wei;HU Qian-qian;WANG Ju-zhong(Emergency Response Office,Center for Disease Control and Prevention of Cangzhou City,Cangzhou Hebei,061000,China;Microbiology Laboratory Department,Center for Disease Control and Prevention of Cangzhou City,Cangzhou Hebei,061000,China)
出处
《职业与健康》
CAS
2023年第3期326-328,共3页
Occupation and Health
基金
沧州市科技支撑计划项目(183302140)。
关键词
水产品
致病性弧菌
污染状况
毒力基因
Aquatic products
Pathogenic vibrio
Pollution status
Virulence genes
