摘要
针对709份花椰菜自交系与骨干亲本,基于亲缘关系与22组农艺性状表型数据,筛选并构建了包含153个品系的核心种质资源集,占比21.58%。根据核心种质的表型变异数据,均值差异百分率为0、极差符合率大于80%以及较高的变异系数变化率等评价指标结果显示,构建的核心种质集的覆盖度与表型代表性良好。核心种质与全部种质之间的表型保留比分析、表型多样性指数t检验、不同性状均值t检验、主成分分析、插入缺失(Insertion/Deletion,InDel)位点计数与分布统计等分析结果进一步验证了该核心种质资源的表型变异特征与遗传多样性,其能够有效代表709份花椰菜原始种质资源。
In the present study,709 inbred lines and backbone parents in cauliflower were resequenced,and their agronomic traits were analyzed.A core collection set containing 153 cauliflower lines was constructed based on the data of genotypes and 22 phenotypes.Furthermore,according to the data of phenotypic variation in these core collection,the results indicated that the percentage of mean difference was zero,the range coincidence rate was higher than 80%,and the variation rate of coefficient of variation was also commomly high.In addition,the analysis,such as phenotypic retention ratio,t test of phenotypic diversity index,t test of mean value of different traits,principal component and statistics of InDel locus counting and distribution,was carried out between the core collection and those original collection.The results confirmed that the core collection population largely represents the phenotypic and genetic diversity of the whole resource,which meets the requirements of core collection construction.
作者
王梦梦
孙德岭
陈锐
杨迎霞
张冠
吕明杰
王倩
谢添羽
牛国保
单晓政
谭津
姚星伟
WANG Mengmeng;SUN Deling;CHEN Rui;YANG Yingxia;ZHANG Guan;LÜMingjie;WANG Qian;XIE Tianyu;NIU Guobao;SHAN Xiaozheng;TAN Jin;YAO Xingwei(Key Laboratory of Food Biotechnology,School of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China;Institute of Germplasm Resources and Biotechnology,Tianjin Academy of Agricultural Sciences,Tianjin 300381,China;Vegetable Research Institute,Tianjin Academy of Agricultural Sciences,Tianjin 300381,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2023年第2期421-431,共11页
Acta Horticulturae Sinica
基金
天津市科技计划项目(19ZXZYSN0050,20YFZCSN00400)
天津市现代农业产业技术体系项目(ITTVRS2017004)
天津市“131”创新型人才团队建设项目(201923)。
关键词
花椰菜
核心种质
表型性状
遗传多样性
cauliflower
core collection
phenotypic trait
genetic diversity
