摘要
以烟草疫霉菌为靶标,从3000株烟草叶片内生细菌中筛选出拮抗菌483株,抑菌率在9.55%~55.96%之间.从这483株拮抗菌中筛选出11株趋化性内生生防细菌,鉴定为Brevibacillus brevis、Br.parabrevis、Br.formosus、Br.reuszeri、Pseudomonas aeruginosa、Ps.tremae、Ps.umsongensis和Erwinia rhapontici.温室试验结果显示,Br.brevis Hs8-19,Br.brevis Hs8-13和Ps.tremae Ht3-25单独使用时,对烟草黑胫病的防效分别为62.43%、57.67%和53.43%.当在菌液中添加终浓度为40μmol/L尼古丁时,Br.brevis Hs8-19,Br.brevis Hs8-13和Ps.tremae Ht3-25对烟草黑胫病的防效分别增加了6.11%、4.79%和4.72%.
A total of 3000 endophytic bacteria were isolated from the healthy leaves of tobacco.Of them,483 antagonistic strains were screened out with Phytophthora nicotianae as the target,and the inhibition rate ranged from 9.55%to 55.96%.From these 483 antagonistic strains,11 chemotactic endophytic bacteria were screened out and were identified as Brevibacillus brevis,Br.formosus,Br.reuszeri,Pseudomonas aeruginosa,Ps.tremae,Ps.umsongensis and Erwinia rhapontici.Under greenhouse condition,endophytic bacteria Br.brevis HS8-19,Br.brevis HS8-13 and Ps.tremae HT3-25 showed their control efficiencies towards tobacco black shank with values of 62.43%,57.67%,and 53.43%,respectively,when used alone.When nicotine was added to the bacterial solution with the concentration of 40μmol/L,the control efficiencies of the three strains were increased by 6.11%,4.79%and 4.72%,respectively.Results of our study indicate that the combination of chemotactic-biocontrol bacteria and chemotactic substances has important potential in the control of tobacco black shank.
作者
韦玉倩
马桂妹
刘剑金
张体坤
王学坚
黄必永
陈增伟
杨学红
秦庆宪
莫明和
刘子仪
WEI Yu-qian;MA Gui-mei;LIU Jian-jin;ZHANG Ti-kun;WANG Xue-jian;HUANG Biyong;CHEN Zeng-wei;YANG Xue-hong;QIN Qing-xian;MO Ming-he;LIU Zi-yi(State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University,Kunming 650091,Yunnan,China;Pu’er Branch of Yunnan Tobacco Company,Pu’er 665000,Yunnan,China)
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2023年第2期531-537,共7页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南省烟草公司科技计划(2018530000241019,2019530000241018)
国家自然科学基金(31960022).
关键词
烟草内生细菌
烟草黑胫病
烟草疫霉菌
趋化性细菌
生物防治
endophytic bacteria of tobacco
tobacco black shank
Phytophthora nicotianae
chemotactic bacteria
biological control
